Exploration of making use of growth-promotion micro organism of Chlorella sorokiniana to open cultivation programs
These days, synthetic development of bacteria-algae consortia to reinforce microalgal biomass is prevalent in enclosed programs, whereas few are in-built an open tradition. On this examine, Achromobacter sp. and Rhizobium sp., remoted from an open pond of Chlorella sorokiniana, had been the microalgal growth-promotion micro organism and chosen to construct the bacteria-algae consortia with axenic C. sorokiniana in open cultivation programs. To look at the efficiency of those two synthetic bacteria-algae consortia in open tradition underneath steady cultivation circumstances, the co-cultivation experiments had been carried out underneath fixed temperature and lightweight depth indoors.
It was discovered that Achromobacter sp. progressively misplaced the dominance of the inhabitants within the co-culture and failed to advertise the expansion of C. sorokiniana throughout open cultivation. Nonetheless, the Rhizobium sp. maintained its dominant inhabitants of bacterial neighborhood in open tradition and will promote the expansion of C. sorokiniana, with an enhancement of 13.76%. To additional consider the results of Rhizobium sp. on microalgae underneath variations of temperature and daylight depth circumstances, the open co-cultivation experiments had been constructed outdoor.
Outcomes confirmed that the expansion of C. sorokiniana may rise 13.29% solely when Rhizobium sp. was added to the tradition repeatedly, and addition of bacterial resolution in log-phase of microalgae may assist Rhizobium sp. dominate within the bacterial neighborhood. On this approach, addition of Rhizobium sp. within the log-phase of C. sorokiniana needs to be an efficient course of to be utilized to open ponds cultivation. Our findings are a step towards making use of growth-promotion micro organism for C. sorokiniana for purposes in open cultivation programs.
Characterization of Two Zygnema Strains ( Zygnema circumcarinatum SAG 698-1a and SAG 698-1b) and a Fast Technique to Estimate Nuclear Genome Dimension of Zygnematophycean Inexperienced Algae
Zygnematophyceae inexperienced algae (ZGA) have been proven to be the closest relations of land crops. Three nuclear genomes of ZGA have been not too long ago printed, and extra genomes are underway. Right here we analyzed two Zygnema circumcarinatum strains SAG 698-1a (mating +) and SAG 698-1b (mating -) and located distinct cell sizes and different morphological variations. The molecular identities of the 2 strains had been additional investigated by sequencing their 18S rRNA, psaA and rbcL genes. These marker genes of SAG 698-1a had been surprisingly far more just like Z. cylindricum (SAG 698-2) than to SAG 698-1b.
Phylogenies of those marker genes additionally confirmed that SAG 698-1a and SAG 698-1b had been effectively separated into two completely different Zygnema clades, the place SAG 698-1a was clustered with Z. cylindricum, whereas SAG 698-1b was clustered with Z. tunetanum. Moreover, physiological parameters like ETRmax values differed between SAG 698-1a and SAG 698-1b after 2 months of cultivation. The de-epoxidation state (DEPS) of the xanthophyll cycle pigments additionally confirmed vital variations. Surprisingly, the 2 strains couldn’t conjugate, and considerably differed within the thickness of the mucilage layer.
Moreover, ZGA cell partitions are extremely enriched with sticky and acidic polysaccharides, and subsequently the extensively used plant nuclear extraction protocols don’t work effectively in ZGA. Right here, we additionally report a quick and easy technique, by mechanical chopping, for environment friendly nuclear extraction within the two SAG strains. Extra importantly, the extracted nuclei had been additional used for nuclear genome dimension estimation of the 2 SAG strains by move cytometry (FC).
) OPTIONAL GREEN FILTER (572NM) |
|
GDBL-GREEN |
CORNING |
1/pk |
EUR 1052 |
|
Description: Lab Equipment; Axygen Branded EQ |
 Antibody for Algae DNAJ |
|
SPC-300B |
Stressmarq |
0.2ml |
EUR 366 |
- DNAJ is a prokaryotic heat shock induced protein from the bacterium E.coli, which is under control of the htpr regulatory protein (1). DNAJ interacts with the chaperone HSP70-like DNAK protein to disassemble a protein complex found at a phage lambda'
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Description: A polyclonal antibody for DNAJ from Algae | Algae (Chlamydomonas reinhardtii) | Bacteria | E. coli (Escherichia coli). The antibody is produced in rabbit after immunization with Algae Recombinant DNAJ of Chlamydomonas reinhardtii. The Antibody is tested and validated for WB, AfP assays with the following recommended dilutions: WB (1:5000). This DNAJ antibody is unconjugated. |
 Antibody for Algae DNAK |
|
SPC-301B |
Stressmarq |
0.2ml |
EUR 366 |
- The prokaryotic HSP70 protein family includes DNA K, HSCA (HSC66), and HSCC (HSC62) (1). DNAK interacts with DNAJ and GrpE heat shock protein chaperones to fold substrate proteins in an ATP-driven cycle (2). These proteins are also involved in cell p
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Description: A polyclonal antibody for DNAK from Algae | Algae (Chlamydomonas reinhardtii). The antibody is produced in rabbit after immunization with Algae Recombinant DNAK of Chlamydomonoas reinhardtii. The Antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:5000). This DNAK antibody is unconjugated. |
) Antibody for Algae HSP70B (Plant) |
|
SPC-315D |
Stressmarq |
0.1ml |
EUR 366 |
- HSP70B (heat shock protein 70B) is a nuclear-encoded, chloroplast-targeted chaperone of the HSP70 family. It is the major HSP70 in the stroma of Chlamydomonas chloroplasts. It interacts with HSP90C, CGE1, CDJ2 and VIPP1 (1-3). It has been shown that
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Description: A polyclonal antibody for HSP70 from Algae | Plant | Algae (Chlamydomonas reinhardtii) | Algae (Desmodesmus subspicatus) | Moss (Physcomitrella patens). The antibody is produced in rabbit after immunization with Algae Mature HSP70B protein expressed with N- and C- terminal hexahistidine tags in E.coli, purified with Ni-NTA. The Antibody is tested and validated for WB, IP assays with the following recommended dilutions: WB (1:10000). This HSP70 antibody is unconjugated. |
) Antibody for Algae HSP90C (Plant) |
|
SPC-317D |
Stressmarq |
0.1ml |
EUR 366 |
- HSP90C is a nuclear-encoded, chloroplast-targeted chaperone of the HSP90 family. In the chloroplast, it is localized to the soluble stroma fraction, but also to thylakoids and low-density membranes containing inner envelopes. HSP90C is expressed unde
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Description: A polyclonal antibody for HSP90 from Algae | Algae (Chlamydomonas reinhardtii). The antibody is produced in rabbit after immunization with Algae C-terminal 238 amino acids of HSP90C expressed with N- and C-terminal hexahistidine tags in E.coli, purified with Ni-NTA.. The Antibody is tested and validated for WB, IP assays with the following recommended dilutions: WB (1:3000). This HSP90 antibody is unconjugated. |
 MitoView Green |
|
70054 |
Biotium |
20X50UG |
EUR 226 |
|
Description: Minimum order quantity: 1 unit of 20X50UG |
 Leucomalachite green |
|
AT105 |
Unibiotest |
1mg |
EUR 1368 |
 Bromocresol green |
|
20-abx186394 |
Abbexa |
|
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- Shipped within 1-2 weeks.
|
Leucomalachite green |
|
AG105 |
Unibiotest |
1 mg |
EUR 523 |
 Brilliant green |
|
BB0242 |
Bio Basic |
25g |
EUR 56.09 |
- Product category: Biochemicals/Indicators/Stains/Biological Stains
|
 Thiazole Green, 10, 000X in DMSO |
|
40086-0.5mL |
Biotium |
0.5mL |
EUR 188 |
|
Description: Minimum order quantity: 1 unit of 0.5mL |
Thiazole Green, 10, 000X in DMSO |
|
40086-1mL |
Biotium |
1mL |
EUR 287 |
|
Description: Minimum order quantity: 1 unit of 1mL |
 ViaVac Red/Green |
|
29068 |
Biotium |
100uL |
EUR 261 |
|
Description: Minimum order quantity: 1 unit of 100uL |
 CytoTell™ Green |
|
22253 |
AAT Bioquest |
500 Tests |
EUR 132 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12352200
|
CytoTell™ Green |
|
22254 |
AAT Bioquest |
1000 Tests |
EUR 176 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12352200
|
 LysoBrite™ Green |
|
22643 |
AAT Bioquest |
500 Tests |
EUR 176 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12352200
|
 Thiolite™ Green |
|
21508 |
AAT Bioquest |
5 mg |
EUR 306 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12352200
|
 Malachite green Antibody |
|
48363-100ul |
SAB |
100ul |
EUR 333 |
Malachite green Antibody |
|
48363-50ul |
SAB |
50ul |
EUR 239 |
 Malachite Green antibody |
|
20-1184 |
Fitzgerald |
100 ul |
EUR 879 |
|
Description: Sheep polyclonal Malachite Green antibody |
 PhosLite™ Green |
|
11630 |
AAT Bioquest |
1 mg |
EUR 219 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12171501
|
 BactoViewâ„¢ Live Green |
|
40102 |
Biotium |
100uL |
EUR 320 |
|
Description: Minimum order quantity: 1 unit of 100uL |
 Leucomalachite green-HRP |
|
80-1192 |
Fitzgerald |
500 ul |
EUR 137 |
|
Description: Leucomalachite green Conjugate for use in immunoassays |
 Malachite green oxalate |
|
abx180007-25g |
Abbexa |
25 g |
EUR 272 |
- Shipped within 1-2 weeks.
|
 C.I.Solvent Green 3 |
|
abx185369-25g |
Abbexa |
25 g |
EUR 509 |
- Shipped within 1-2 weeks.
|
 Green qPCR SuperMix |
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20-abx098031 |
Abbexa |
-
EUR 1038.00
-
EUR 439.00
-
EUR 634.00
|
|
- Shipped within 5-10 working days.
|
 Fast Green FCF |
|
abx082188-5g |
Abbexa |
5 g |
EUR 203 |
- Shipped within 5-10 working days.
|
) Indocyanine green(Cardiogreen) |
|
E1KS1924 |
EnoGene |
50 mg |
EUR 247 |
 Green Fluorescent Protein |
|
CH22124 |
Neuromics |
100 ul of 1 mg/ml |
EUR 435 |
 Leuco- Malachiet green |
|
AG131 |
Unibiotest |
1 mg |
EUR 523 |
 Safe-Green™ |
|
G108-G |
ABM |
1.0 ml |
EUR 71 |
 Green Taq Mix |
|
P131-01 |
Vazyme |
5 ml |
EUR 119 |
Green Taq Mix |
|
P131-02 |
Vazyme |
15 ml |
EUR 150 |
Green Taq Mix |
|
P131-03 |
Vazyme |
50 ml |
EUR 248 |
Green Fluorescent Protein |
|
MO22184 |
Neuromics |
100 ul |
EUR 409 |
 0.6ML MICROTUBES. GREEN |
|
MCT-060-G |
CORNING |
1000/pk |
EUR 183 |
|
Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
 1.7ML MICROTUBES. GREEN |
|
MCT-175-G |
CORNING |
500/pk |
EUR 116 |
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Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
 Accuris qMax Green |
|
PR2000-H-100 |
BenchMark |
1 PC |
EUR 115.24 |
- To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
|
Accuris qMax Green |
|
PR2000-H-1000 |
BenchMark |
1 PC |
EUR 467.74 |
- To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
|
Accuris qMax Green |
|
PR2000-H-500 |
BenchMark |
1 PC |
EUR 284.31 |
- To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
|
Accuris qMax Green |
|
PR2000-H-S |
BenchMark |
1 PC |
EUR 73.77 |
- To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
|
Accuris qMax Green |
|
PR2000-L-100 |
BenchMark |
1 PC |
EUR 115.24 |
- To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
|
Accuris qMax Green |
|
PR2000-L-1000 |
BenchMark |
1 PC |
EUR 467.74 |
- To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
|
Accuris qMax Green |
|
PR2000-L-500 |
BenchMark |
1 PC |
EUR 284.31 |
- To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
|
Accuris qMax Green |
|
PR2000-L-S |
BenchMark |
1 PC |
EUR 73.77 |
- To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
|
Accuris qMax Green |
|
PR2000-N-100 |
BenchMark |
1 PC |
EUR 113.65 |
- To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
|
Accuris qMax Green |
|
PR2000-N-1000 |
BenchMark |
1 PC |
EUR 459.76 |
- To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
|
Accuris qMax Green |
|
PR2000-N-500 |
BenchMark |
1 PC |
EUR 279.53 |
- To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
|
Accuris qMax Green |
|
PR2000-N-S |
BenchMark |
1 PC |
EUR 72.98 |
- To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
|
 ViSafe Green Gel |
|
S435 |
GeneOn |
500 µl |
EUR 146 |
ViSafe Green Gel |
|
S435L |
GeneOn |
5x500 µl |
EUR 557 |
Fast Green FCF |
|
FB0452 |
Bio Basic |
5g |
EUR 55.22 |
- Product category: Biochemicals/Indicators/Stains/Peptide/Protein Related
|
Malachite green oxalate |
|
MD0330 |
Bio Basic |
50g |
EUR 57.83 |
- Product category: Biochemicals/Indicators/Stains/Biological Stains
|
 Green-DNA Dye |
|
SB2040 |
Bio Basic |
100UL, 100UL |
EUR 67.4 |
- Product category: Electrophoresis Related/Tracking Dyes
|
 recombinant expressed in yeast) Insulin (human) recombinant expressed in yeast |
|
B7407-10 |
ApexBio |
10 mg |
EUR 145 |
|
Description: IC50: 5.01 nmol/L for insulin receptorInsulin is a peptide hormone produced by beta cells in pancreas. Insulin has been found to stimulate glucose uptake into muscle and adipose tissue, which is central to the maintenance of whole body glucose homeostasis. |
 MitoLite™ Green EX488 |
|
22675 |
AAT Bioquest |
500 Tests |
EUR 132 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12352200
|
 MitoLite™ Green FM |
|
22695 |
AAT Bioquest |
10x50 ug |
EUR 176 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12352200
|
 MycoLight™ Green JJ98 |
|
24000 |
AAT Bioquest |
100 ul |
EUR 219 |
- R-phrase: R23, R24, R25
- H-Phrase: H301, H311, H331
- Symbol for dangerous compounds: T
- UNSPEC Code: 12352200
|
 MycoLight™ Green JJ99 |
|
24001 |
AAT Bioquest |
100 ul |
EUR 219 |
- R-phrase: R23, R24, R25
- H-Phrase: H301, H311, H331
- Symbol for dangerous compounds: T
- UNSPEC Code: 12352200
|
 Protonex™ Green 500 |
|
21215 |
AAT Bioquest |
1 mg |
EUR 219 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12352200
|
 Recombinant Green Fluorescent Protein |
|
7-06112 |
CHI Scientific |
10µg |
Ask for price |
Recombinant Green Fluorescent Protein |
|
7-06113 |
CHI Scientific |
50µg |
Ask for price |
Recombinant Green Fluorescent Protein |
|
7-06114 |
CHI Scientific |
1mg |
Ask for price |
 MitoView Green, Trial Size |
|
70054-T |
Biotium |
50UG |
EUR 94 |
|
Description: Minimum order quantity: 1 unit of 50UG |
 MMP Green™ substrate |
|
13520 |
AAT Bioquest |
1 mg |
EUR 480 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12352200
|
 Nuclear Green™ LCS1 |
|
17540 |
AAT Bioquest |
0.5 ml |
EUR 132 |
- R-phrase: R20, R21, R68
- H-Phrase: H303, H313, H340
- Symbol for dangerous compounds: T
- UNSPEC Code: 41116134
|
 Nuclear Green™ DCS1 |
|
17550 |
AAT Bioquest |
0.5 ml |
EUR 132 |
- R-phrase: R20, R21, R68
- H-Phrase: H303, H313, H340
- Symbol for dangerous compounds: T
- UNSPEC Code: 41116134
|
 EZSolution? SYBR Green I |
|
B1748-1 |
Biovision |
|
EUR 620 |
EZSolution? SYBR Green I |
|
B1748-500 |
Biovision |
|
EUR 430 |
 Malachite Green ELISA Kit |
|
DEIA057 |
Creative Diagnostics |
96T |
EUR 1251 |
|
Description: The Malachite Green ELISA Kit is a direct competitive enzyme immunoassay for the quantitative analysis of Malachite green in fish and shrimp samples. |
 Green qPCR SuperMix UDG |
|
20-abx098032 |
Abbexa |
-
EUR 1135.00
-
EUR 453.00
-
EUR 662.00
|
|
- Shipped within 5-10 working days.
|
 Top Green qPCR SuperMix |
|
20-abx098033 |
Abbexa |
-
EUR 829.00
-
EUR 217.00
-
EUR 1135.00
-
EUR 411.00
|
|
- Shipped within 5-10 working days.
|
 Tip Green qPCR SuperMix |
|
20-abx098034 |
Abbexa |
-
EUR 968.00
-
EUR 230.00
-
EUR 1330.00
-
EUR 467.00
|
|
- Shipped within 5-10 working days.
|
) Green Fluorescent Protein (GFP) |
|
CH23105 |
Neuromics |
400 ul |
EUR 761 |
 Malachite green Conjugated Antibody |
|
C48363 |
SAB |
100ul |
EUR 397 |
 DNA Loading Green Dye |
|
G924 |
ABM |
6X; 3 x 1.0 ml |
EUR 79 |
 Green Lysis Kit 50 |
|
GREENE1 |
Next Advance |
50pack |
EUR 156 |
|
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. |
 Green Lysis Kit 250 |
|
GREENE5 |
Next Advance |
250pack |
EUR 468 |
|
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. |
 Polycaspase Assay Kit, green |
|
KF17200 |
Neuromics |
25 Tests |
EUR 304 |
) Novel Green PLUS (20000X) |
|
LD003-0500 |
GeneOn |
500 µl |
EUR 172 |
Novel Green PLUS (20000X) |
|
LD003-0500L |
GeneOn |
5x500 µl |
EUR 731 |
To verify the FC end result, we’ve got additionally used different experimental strategies for nuclear genome dimension estimation of the 2 strains. Curiously, the 2 strains had been discovered to have very distinct nuclear genome sizes. Our a number of traces of proof strongly point out that SAG 698-1a presumably had been confused with SAG 698-2 previous to 2005, and almost definitely represents Z. cylindricum or a intently associated species.
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