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Chromochloris zofingiensis (Chlorophyceae) Divides by Consecutive Multiple Fission Cell-Cycle under Batch and Continuous Cultivation
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Chromochloris zofingiensis (Chlorophyceae) Divides by Consecutive Multiple Fission Cell-Cycle under Batch and Continuous Cultivation

A number of inexperienced algae can divide by a number of fission and spontaneously synchronize their cell cycle with the obtainable mild regime. The yields that may be obtained from a microalgal tradition are instantly affected by cell cycle occasions. Chromochloris zofingiensis is taken into account as one of the crucial promising microalgae for biotechnological purposes because of its quick progress and the versatile trophic capabilities. It’s intensively investigated within the context of bio-commodities manufacturing (carotenoids, storage lipids); nonetheless, the sample of cell-cycle occasions underneath frequent cultivation methods was not but characterised for C. zofingiensis.

Rabbit Anti BSA
EnoGene
RABBIT ANTI HRK
MyBiosource
RABBIT ANTI HRK
MyBiosource
RABBIT ANTI BIM
MyBiosource
RABBIT ANTI BIM
MyBiosource

On this examine, we’ve got employed fluorescence microscopy to characterize the fundamental cell-cycle dynamics underneath batch and steady modes of phototrophic C. zofingiensis cultivation. Staining with SYBR green-applied in DMSO solution-enabled, for the primary time, the clear and easy visualization of polynuclear phases on this microalga. Accordingly, we concluded that C. zofingiensis divides by a consecutive sample of a number of fission, whereby it spontaneously synchronizes progress and cell division in keeping with the obtainable illumination regime.

In high-light steady tradition or low-light batch tradition, C. zofingiensis cell-cycle was accomplished inside a number of light-dark (L/D) cycles (14 h/10 h); nonetheless, cell divisions had been synchronized with the darkish intervals solely within the high-light steady tradition. In each modes of cultivation, daughter cell launch was primarily facilitated by division of eight and 16-polynuclear cells. The outcomes of this examine are of each elementary and utilized science significance and are additionally necessary for the event of an environment friendly nuclear transformation system for C. zofingiensis.

MARK Antibody
AAT Bioquest
MARK Antibody
SAB
MARK Antibody
SAB
MARK antibody
Fitzgerald
MARK antibody
Fitzgerald

Exploration of making use of growth-promotion micro organism of Chlorella sorokiniana to open cultivation programs

These days, synthetic development of bacteria-algae consortia to reinforce microalgal biomass is prevalent in enclosed programs, whereas few are in-built an open tradition. On this examine, Achromobacter sp. and Rhizobium sp., remoted from an open pond of Chlorella sorokiniana, had been the microalgal growth-promotion micro organism and chosen to construct the bacteria-algae consortia with axenic C. sorokiniana in open cultivation programs. To look at the efficiency of those two synthetic bacteria-algae consortia in open tradition underneath steady cultivation circumstances, the co-cultivation experiments had been carried out underneath fixed temperature and lightweight depth indoors.

It was discovered that Achromobacter sp. progressively misplaced the dominance of the inhabitants within the co-culture and failed to advertise the expansion of C. sorokiniana throughout open cultivation. Nonetheless, the Rhizobium sp. maintained its dominant inhabitants of bacterial neighborhood in open tradition and will promote the expansion of C. sorokiniana, with an enhancement of 13.76%. To additional consider the results of Rhizobium sp. on microalgae underneath variations of temperature and daylight depth circumstances, the open co-cultivation experiments had been constructed outdoor.

Outcomes confirmed that the expansion of C. sorokiniana may rise 13.29% solely when Rhizobium sp. was added to the tradition repeatedly, and addition of bacterial resolution in log-phase of microalgae may assist Rhizobium sp. dominate within the bacterial neighborhood. On this approach, addition of Rhizobium sp. within the log-phase of C. sorokiniana needs to be an efficient course of to be utilized to open ponds cultivation. Our findings are a step towards making use of growth-promotion micro organism for C. sorokiniana for purposes in open cultivation programs.

Recombinant Humanp21 Recombinant Protein
ProSci
TWEAK, recombinant / TNFSF12, recombinant (Human)
PHOENIX PEPTIDE
TAGLN Recombinant Protein (Rat) (Recombinant- Tag)
ABM
TAGLN2 Recombinant Protein (Rat) (Recombinant Tag)
ABM
TAGLN3 Recombinant Protein (Rat) (Recombinant Tag)
ABM

Chromochloris zofingiensis (Chlorophyceae) Divides by Consecutive Multiple Fission Cell-Cycle under Batch and Continuous Cultivation

Characterization of Two Zygnema Strains ( Zygnema circumcarinatum SAG 698-1a and SAG 698-1b) and a Fast Technique to Estimate Nuclear Genome Dimension of Zygnematophycean Inexperienced Algae

Zygnematophyceae inexperienced algae (ZGA) have been proven to be the closest relations of land crops. Three nuclear genomes of ZGA have been not too long ago printed, and extra genomes are underway. Right here we analyzed two Zygnema circumcarinatum strains SAG 698-1a (mating +) and SAG 698-1b (mating -) and located distinct cell sizes and different morphological variations. The molecular identities of the 2 strains had been additional investigated by sequencing their 18S rRNA, psaA and rbcL genes. These marker genes of SAG 698-1a had been surprisingly far more just like Z. cylindricum (SAG 698-2) than to SAG 698-1b.

Phylogenies of those marker genes additionally confirmed that SAG 698-1a and SAG 698-1b had been effectively separated into two completely different Zygnema clades, the place SAG 698-1a was clustered with Z. cylindricum, whereas SAG 698-1b was clustered with Z. tunetanum. Moreover, physiological parameters like ETRmax values differed between SAG 698-1a and SAG 698-1b after 2 months of cultivation. The de-epoxidation state (DEPS) of the xanthophyll cycle pigments additionally confirmed vital variations. Surprisingly, the 2 strains couldn’t conjugate, and considerably differed within the thickness of the mucilage layer.

Recombinant Humanp21 Recombinant Protein
92-035
TWEAK, recombinant / TNFSF12, recombinant (Human)
054-85
TAGLN Recombinant Protein (Rat) (Recombinant- Tag)
RP232205
TAGLN2 Recombinant Protein (Rat) (Recombinant Tag)
RP232208
TAGLN3 Recombinant Protein (Rat) (Recombinant Tag)
RP232211

Moreover, ZGA cell partitions are extremely enriched with sticky and acidic polysaccharides, and subsequently the extensively used plant nuclear extraction protocols don’t work effectively in ZGA. Right here, we additionally report a quick and easy technique, by mechanical chopping, for environment friendly nuclear extraction within the two SAG strains. Extra importantly, the extracted nuclei had been additional used for nuclear genome dimension estimation of the 2 SAG strains by move cytometry (FC).

Thermostable Human Basic Fibroblast Growth Factor, Expressed In Green Algae
MBS430536-001mg 0.01mg
EUR 235

Thermostable Human Basic Fibroblast Growth Factor, Expressed In Green Algae
MBS430536-5x001mg 5x0.01mg
EUR 915

Thermostable Human Basic Fibroblast Growth Factor, Expressed In Green Algae
MBS430537-005mg 0.05mg
EUR 560

Thermostable Human Basic Fibroblast Growth Factor, Expressed In Green Algae
MBS430537-5x005mg 5x0.05mg
EUR 2365

Green Algae Lysate
PABL-1306 50 ug
EUR 196.8

GC (green control baculovirus expressing GFP)
C14 1 ml
EUR 195
Description: Protein expression

GCP (green control plasmid for GFP expression)
C15 50 ul
EUR 145
Description: Protein expression

Express Cloning Checker Kit II: Green Solution
K5012100-1 1 ml
EUR 75

Ribonuclease H, expressed in E. coli
GZ5072 250U
EUR 427.37

Ribonuclease H, expressed in E. coli
GZ5072-1 1
EUR 466.8

Ribonuclease H, expressed in E. coli
GZ5072-1KU 1 KU
EUR 600

Ribonuclease H, expressed in E. coli
GZ5072-250 250
EUR 182

Ribonuclease H, expressed in E. coli
GZ5072-250U 250 U
EUR 255.6

T4 DNA Ligase, expressed in E. coli
GZ9900 1KIU
EUR 398.33

T4 DNA Ligase, expressed in E. coli
GZ9900-1 1
EUR 150.4

T4 DNA Ligase, expressed in E. coli
GZ9900-1KIU 1 KIU
EUR 217.2

T4 DNA Ligase, expressed in E. coli
GZ9900-5 5
EUR 435.1

T4 DNA Ligase, expressed in E. coli
GZ9900-5KIU 5 KIU
EUR 562.8

PNGase F, Recombinant (Expressed in E.coli)
RK20565 100000U
EUR 65.4

PNGase F, Recombinant (Expressed in Yeast)
RK20564 20000U
EUR 256.15

Reverse Transcriptase, expressed in E. coli
GZ8080 10KIU
EUR 463.47

Reverse Transcriptase, expressed in E. coli
GZ8080-10 10
EUR 253.1

Reverse Transcriptase, expressed in E. coli
GZ8080-10KIU 10 KIU
EUR 342

Reverse Transcriptase, expressed in E. coli
GZ8080-25 25
EUR 506.2

Reverse Transcriptase, expressed in E. coli
GZ8080-25KIU 25 KIU
EUR 648

Insulin (human) recombinant expressed in yeast
B7407-10 10 mg
EUR 40
Description: Endogenous insulin receptor agonist

Algae Culture Agar
M343-500G 1 unit
EUR 45.2
Description: Algae Culture Agar

Algae Culture Agar
36191 100 Gms
EUR 8.55
Description: Part D

Algae Culture Agar
36191-1 500 Gms
EUR 34.21
Description: Part D

rec Human Growth Hormone (expressed in E.coli)
H-3148.0500 0.5mg
EUR 1096.8

rec Human Growth Hormone (expressed in E.coli)
H-3148.1000 1.0mg
EUR 1936.8

Algae Culture Broth
M342-100G 1 unit
EUR 14.77
Description: Algae Culture Broth

Algae Culture Broth
M342-500G 1 unit
EUR 42.8
Description: Algae Culture Broth

Algae Culture Broth
72439 100 Gms
EUR 7.7
Description: Part D

Algae Culture Broth
72439-1 500 Gms
EUR 33.36
Description: Part D

rec HIV-1 Protease (expressed in E. coli)
4030739.005 50 µg
EUR 654.99

rec HIV-1 Protease (expressed in E. coli)
4030739.01 0.1 mg
EUR 1114.47

rec HIV-1 Protease (expressed in E. coli)
H-9040.0050 50.0µg
EUR 1051.2

rec HIV-1 Protease (expressed in E. coli)
H-9040.0100 0.1mg
EUR 1762.8

rec HIV-1 Protease (expressed in E. coli)
H-9040.0500 0.5mg
EUR 6925.2

Melanoma antigen preferentially expressed in tumors
AP87209 1mg
EUR 2640

rec Human Growth Hormone (expressed in E. coli)
4030632.05 0.5 mg
EUR 684.18

rec Human Growth Hormone (expressed in E. coli)
4030632.1 1 mg
EUR 1226.82

Highly Expressed In Cancer Protein (HEC1) Antibody
abx432790-200ul 200 ul
EUR 460.8

Highly Expressed In Cancer Protein (HEC1) Antibody
abx432790-100g 100 µg
EUR 387.5

Zip4H (Tex11, Testis expressed 11, testis expressed sequence 11, TGC1, TSGA3)
MBS608667-01mg 0.1mg
EUR 745

Zip4H (Tex11, Testis expressed 11, testis expressed sequence 11, TGC1, TSGA3)
MBS608667-5x01mg 5x0.1mg
EUR 3195

Zip4H (Tex11, Testis Expressed 11, Testis Expressed Sequence 11, TGC1, TSGA3)
MBS624391-01mg 0.1mg
EUR 520

Zip4H (Tex11, Testis Expressed 11, Testis Expressed Sequence 11, TGC1, TSGA3)
MBS624391-5x01mg 5x0.1mg
EUR 2185

Zip4H (Tex11, Testis Expressed 11, Testis Expressed Sequence 11, TGC1, TSGA3)
MBS626530-01mg 0.1mg
EUR 875

Zip4H (Tex11, Testis Expressed 11, Testis Expressed Sequence 11, TGC1, TSGA3)
MBS626530-5x01mg 5x0.1mg
EUR 3790

Homeobox Expressed In ES Cells 1 (HESX1) Antibody
abx233841-100ug 100 ug
EUR 577.2

Homeobox Expressed In ES Cells 1 (HESX1) Antibody
20-abx311073
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Homeobox Expressed In ES Cells 1 (HESX1) Antibody
abx233841-100g 100 µg
EUR 350

Homeobox Expressed In ES Cells 1 (HESX1) Antibody
abx375555-96tests 96 tests
EUR 337.5

Homeobox Expressed In ES Cells 1 (HESX1) Antibody
abx311073-100g 100 µg
EUR 362.5

Homeobox Expressed In ES Cells 1 (HESX1) Antibody
abx311073-20g 20 µg
EUR 162.5

Homeobox Expressed In ES Cells 1 (HESX1) Antibody
abx311073-50g 50 µg
EUR 250

Gene Differentially Expressed In Prostate (GDEP) Antibody
abx233402-100g 100 µg
EUR 350

Homeobox Expressed In ES Cells 1 (HESX1) Antibody (HRP)
20-abx311074
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
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Homeobox Expressed In ES Cells 1 (HESX1) Antibody (HRP)
abx311074-100g 100 µg
EUR 362.5

Homeobox Expressed In ES Cells 1 (HESX1) Antibody (HRP)
abx311074-20g 20 µg
EUR 162.5

Homeobox Expressed In ES Cells 1 (HESX1) Antibody (HRP)
abx311074-50g 50 µg
EUR 250

Non-Metastatic Cells 3, Protein Expressed In Antibody
20-abx114145
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  • 150 ul
  • 50 ul

Non-Metastatic Cells 3, Protein Expressed In Antibody
abx114145-100l 100 µl
EUR 612.5

Preferentially Expressed Antigen In Melanoma (PRAME) Antibody
20-abx104373
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  • 100 ug
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Preferentially Expressed Antigen In Melanoma (PRAME) Antibody
20-abx104374
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  • 100 ug
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Homeobox Expressed In ES Cells 1 (HESX1) Antibody (FITC)
20-abx311075
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  • 100 ug
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  • 200 ug
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Homeobox Expressed In ES Cells 1 (HESX1) Antibody (FITC)
abx311075-100g 100 µg
EUR 362.5

Homeobox Expressed In ES Cells 1 (HESX1) Antibody (FITC)
abx311075-20g 20 µg
EUR 162.5

Homeobox Expressed In ES Cells 1 (HESX1) Antibody (FITC)
abx311075-50g 50 µg
EUR 250

Melanoma antigen preferentially expressed in tumors Antibody
20-abx109324
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  • 100 ug
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Differentially Expressed In FDCP 6 Homolog (DEF6) Antibody
20-abx112066
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  • 150 ul
  • 50 ul

Amy1 Associated Protein Expressed In Testis (AAT1) Antibody
20-abx129058
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  • 100 ug
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Differentially Expressed In FDCP 6 Homolog (DEF6) Antibody
abx028902-400ul 400 ul
EUR 627.6

Differentially Expressed In FDCP 6 Homolog (DEF6) Antibody
abx028902-80l 80 µl
EUR 343.2

Albumin, Human, Recombinant expressed in Rice, Animal-Free
12880-81 1G
EUR 70

Albumin, Human, Recombinant expressed in Rice, Animal-Free
19597-01 1G
EUR 70

Albumin, Human, Recombinant expressed in Rice, Animal-Free
19597-14 5G
EUR 301

Differentially Expressed In FDCP 6 Homolog (DEF6) Antibody
abx232325-100ug 100 ug
EUR 577.2

Differentially Expressed In FDCP 8 Homolog (DEF8) Antibody
20-abx308147
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  • 100 ug
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Differentially Expressed In FDCP 6 Homolog (DEF6) Antibody
abx430013-200ul 200 ul
EUR 460.8

Differentially Expressed In FDCP 6 Homolog (DEF6) Antibody
20-abx318077
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  • 100 ug
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Amy1 Associated Protein Expressed In Testis (AAT1) Antibody
abx129058-100l 100 µl
EUR 287.5

Amy1 Associated Protein Expressed In Testis (AAT1) Antibody
abx129058-1ml 1 ml
EUR 825

Amy1 Associated Protein Expressed In Testis (AAT1) Antibody
abx129058-200l 200 µl
EUR 362.5

Differentially Expressed In FDCP 6 Homolog (DEF6) Antibody
abx112066-100l 100 µl
EUR 612.5

Differentially Expressed In FDCP 8 Homolog (DEF8) Antibody
abx027666-400l 400 µl
EUR 518.75

Differentially Expressed In FDCP 6 Homolog (DEF6) Antibody
abx028902-400l 400 µl
EUR 518.75

Differentially Expressed In FDCP 6 Homolog (DEF6) Antibody
abx232325-100g 100 µg
EUR 350

Differentially Expressed In FDCP 6 Homolog (DEF6) Antibody
abx430013-200l 200 µl
EUR 387.5

Differentially Expressed In FDCP 6 Homolog (DEF6) Antibody
abx376131-96tests 96 tests
EUR 337.5

Differentially Expressed In FDCP 6 Homolog (DEF6) Antibody
abx318077-100g 100 µg
EUR 362.5

Differentially Expressed In FDCP 6 Homolog (DEF6) Antibody
abx318077-20g 20 µg
EUR 162.5

Differentially Expressed In FDCP 6 Homolog (DEF6) Antibody
abx318077-50g 50 µg
EUR 250

Differentially Expressed In FDCP 8 Homolog (DEF8) Antibody
abx308147-100g 100 µg
EUR 362.5

Differentially Expressed In FDCP 8 Homolog (DEF8) Antibody
abx308147-20g 20 µg
EUR 162.5

Differentially Expressed In FDCP 8 Homolog (DEF8) Antibody
abx308147-50g 50 µg
EUR 250

Sclerostin Mammalian Expressed
MBS343290-001mg 0.01mg
EUR 235

Sclerostin Mammalian Expressed
MBS343290-01mg 0.1mg
EUR 560

Sclerostin Mammalian Expressed
MBS343290-1mg 1mg
EUR 3135

Sclerostin Mammalian Expressed
MBS343290-5x1mg 5x1mg
EUR 13915

Homeobox Expressed In ES Cells 1 (HESX1) Antibody (Biotin)
20-abx311076
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Homeobox Expressed In ES Cells 1 (HESX1) Antibody (Biotin)
abx311076-100g 100 µg
EUR 362.5

Homeobox Expressed In ES Cells 1 (HESX1) Antibody (Biotin)
abx311076-20g 20 µg
EUR 162.5

Homeobox Expressed In ES Cells 1 (HESX1) Antibody (Biotin)
abx311076-50g 50 µg
EUR 250

Recombinant Human ACE2 Fc Protein expressed in CHO-K1
MBS669488-005mg 0.05mg
EUR 410

Recombinant Human ACE2 Fc Protein expressed in CHO-K1
MBS669488-01mg 0.1mg
EUR 590

Recombinant Human ACE2 Fc Protein expressed in CHO-K1
MBS669488-05mg 0.5mg
EUR 1975

Recombinant Human ACE2 Fc Protein expressed in CHO-K1
MBS669488-5x05mg 5x0.5mg
EUR 8880

Insulin, Human, Recombinant expressed in Yeast, Animal-Free
12878-44 250MG
EUR 234.5

Insulin, Human, Recombinant expressed in Yeast, Animal-Free
12878-86 50MG
EUR 63

Rat Preferentially Expressed Antigen In Melanoma ELISA Kit
MBS107641-10x96StripWells 10x96-Strip-Wells
EUR 6725

Rat Preferentially Expressed Antigen In Melanoma ELISA Kit
MBS107641-48StripWells 48-Strip-Wells
EUR 550

Rat Preferentially Expressed Antigen In Melanoma ELISA Kit
MBS107641-5x96StripWells 5x96-Strip-Wells
EUR 3420

Rat Preferentially Expressed Antigen In Melanoma ELISA Kit
MBS107641-96StripWells 96-Strip-Wells
EUR 765

Cat Preferentially Expressed Antigen In Melanoma ELISA Kit
MBS063397-INQUIRE INQUIRE Ask for price

Recombinant Mouse Differentially expressed in FDCP 8 (Def8)
MBS1415075-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1315

Recombinant Mouse Differentially expressed in FDCP 8 (Def8)
MBS1415075-002mgEColi 0.02mg(E-Coli)
EUR 1005

Recombinant Mouse Differentially expressed in FDCP 8 (Def8)
MBS1415075-002mgYeast 0.02mg(Yeast)
EUR 1110

Recombinant Mouse Differentially expressed in FDCP 8 (Def8)
MBS1415075-01mgEColi 0.1mg(E-Coli)
EUR 1170

Recombinant Mouse Differentially expressed in FDCP 8 (Def8)
MBS1415075-01mgYeast 0.1mg(Yeast)
EUR 1265

Recombinant Mouse Differentially expressed in FDCP 6 (Def6)
MBS1435161-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1485

Recombinant Mouse Differentially expressed in FDCP 6 (Def6)
MBS1435161-002mgEColi 0.02mg(E-Coli)
EUR 1190

Recombinant Mouse Differentially expressed in FDCP 6 (Def6)
MBS1435161-002mgYeast 0.02mg(Yeast)
EUR 1245

Recombinant Mouse Differentially expressed in FDCP 6 (Def6)
MBS1435161-01mgEColi 0.1mg(E-Coli)
EUR 1435

Recombinant Mouse Differentially expressed in FDCP 6 (Def6)
MBS1435161-01mgYeast 0.1mg(Yeast)
EUR 1465

Testis Expressed 101 (TEX101) Antibody
abx238605-100ug 100 ug
EUR 661.2

Testis Expressed 264 (TEX264) Antibody
abx238611-100ug 100 ug
EUR 610.8

Testis Expressed 101 (TEX101) Antibody
abx238605-100l 100 µl Ask for price

Testis Expressed 101 (TEX101) Antibody
abx238605-50l 50 µl
EUR 350

Testis Expressed 264 (TEX264) Antibody
abx238611-100l 100 µl Ask for price

Testis Expressed 264 (TEX264) Antibody
abx238611-50l 50 µl
EUR 350

Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)
RPH323Hu01 10ug
EUR 190

Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)
4-RPH323Hu01
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  • 5 mg
Description: Recombinant Human Preferentially Expressed Antigen In Melanoma expressed in: E.coli

Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)
RPH323Hu02 10ug
EUR 190

Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)
4-RPH323Hu02
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  • 100 ug
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  • 5 mg
Description: Recombinant Human Preferentially Expressed Antigen In Melanoma expressed in: E.coli

Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)
RPU43399-100ug 100ug
EUR 586.3

Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)
RPU43399-1mg 1mg
EUR 2598.7

Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)
RPU43399-50ug 50ug
EUR 470.8

Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)
RPU43400-100ug 100ug
EUR 586.3

Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)
RPU43400-1mg 1mg
EUR 2598.7

To verify the FC end result, we’ve got additionally used different experimental strategies for nuclear genome dimension estimation of the 2 strains. Curiously, the 2 strains had been discovered to have very distinct nuclear genome sizes. Our a number of traces of proof strongly point out that SAG 698-1a presumably had been confused with SAG 698-2 previous to 2005, and almost definitely represents Z. cylindricum or a intently associated species.

anti- Antibody^Polyclonal antibody control antibody
LSMab09882
H2B Antibody Antibody
E11-184659
Lck antibody Antibody
GWB-250026
H2B Antibody Antibody
MBS8529199-01mg
H2B Antibody Antibody
MBS8529199-01mLAF405L

 

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