Chromochloris zofingiensis (Chlorophyceae) Divides by Consecutive Multiple Fission Cell-Cycle under Batch and Continuous Cultivation

Chromochloris zofingiensis (Chlorophyceae) Divides by Consecutive Multiple Fission Cell-Cycle under Batch and Continuous Cultivation

A number of inexperienced algae can divide by a number of fission and spontaneously synchronize their cell cycle with the obtainable mild regime. The yields that may be obtained from a microalgal tradition are instantly affected by cell cycle occasions. Chromochloris zofingiensis is taken into account as one of the crucial promising microalgae for biotechnological purposes because of its quick progress and the versatile trophic capabilities. It’s intensively investigated within the context of bio-commodities manufacturing (carotenoids, storage lipids); nonetheless, the sample of cell-cycle occasions underneath frequent cultivation methods was not but characterised for C. zofingiensis.

Rabbit Polyclonal antibody Anti-CRBN
ImmunoStep
Polyclonal Goat anti-GST α-form
Detroit R&D
Polyclonal Goat anti-GST μ-form
Detroit R&D
Polyclonal Goat anti-GST p-form
Detroit R&D
SensiTek Anti-Rabbit
ScyTek Laboratories

On this examine, we’ve got employed fluorescence microscopy to characterize the fundamental cell-cycle dynamics underneath batch and steady modes of phototrophic C. zofingiensis cultivation. Staining with SYBR green-applied in DMSO solution-enabled, for the primary time, the clear and easy visualization of polynuclear phases on this microalga. Accordingly, we concluded that C. zofingiensis divides by a consecutive sample of a number of fission, whereby it spontaneously synchronizes progress and cell division in keeping with the obtainable illumination regime.

In high-light steady tradition or low-light batch tradition, C. zofingiensis cell-cycle was accomplished inside a number of light-dark (L/D) cycles (14 h/10 h); nonetheless, cell divisions had been synchronized with the darkish intervals solely within the high-light steady tradition. In each modes of cultivation, daughter cell launch was primarily facilitated by division of eight and 16-polynuclear cells. The outcomes of this examine are of each elementary and utilized science significance and are additionally necessary for the event of an environment friendly nuclear transformation system for C. zofingiensis.

MARK antibody
Fitzgerald
MARK antibody
Fitzgerald
MARK Antibody
SAB
MARK Antibody
SAB
MARK Antibody
Lifescience Market

Exploration of making use of growth-promotion micro organism of Chlorella sorokiniana to open cultivation programs

These days, synthetic development of bacteria-algae consortia to reinforce microalgal biomass is prevalent in enclosed programs, whereas few are in-built an open tradition. On this examine, Achromobacter sp. and Rhizobium sp., remoted from an open pond of Chlorella sorokiniana, had been the microalgal growth-promotion micro organism and chosen to construct the bacteria-algae consortia with axenic C. sorokiniana in open cultivation programs. To look at the efficiency of those two synthetic bacteria-algae consortia in open tradition underneath steady cultivation circumstances, the co-cultivation experiments had been carried out underneath fixed temperature and lightweight depth indoors.

It was discovered that Achromobacter sp. progressively misplaced the dominance of the inhabitants within the co-culture and failed to advertise the expansion of C. sorokiniana throughout open cultivation. Nonetheless, the Rhizobium sp. maintained its dominant inhabitants of bacterial neighborhood in open tradition and will promote the expansion of C. sorokiniana, with an enhancement of 13.76%. To additional consider the results of Rhizobium sp. on microalgae underneath variations of temperature and daylight depth circumstances, the open co-cultivation experiments had been constructed outdoor.

Outcomes confirmed that the expansion of C. sorokiniana may rise 13.29% solely when Rhizobium sp. was added to the tradition repeatedly, and addition of bacterial resolution in log-phase of microalgae may assist Rhizobium sp. dominate within the bacterial neighborhood. On this approach, addition of Rhizobium sp. within the log-phase of C. sorokiniana needs to be an efficient course of to be utilized to open ponds cultivation. Our findings are a step towards making use of growth-promotion micro organism for C. sorokiniana for purposes in open cultivation programs.

Recombinant Humanp21 Recombinant Protein
ProSci
Recombinant phosphopantetheine adenylyltransferase (PPAT) (Recombinant)
Abbexa
Glyco Recombinant Protein A33 Recombinant Protein
ProSci
Glyco Recombinant Protein A33 Recombinant Protein
ProSci
STAG3L1 Recombinant Protein (Human) (Recombinant Tag)
ABM

Chromochloris zofingiensis (Chlorophyceae) Divides by Consecutive Multiple Fission Cell-Cycle under Batch and Continuous Cultivation

Characterization of Two Zygnema Strains ( Zygnema circumcarinatum SAG 698-1a and SAG 698-1b) and a Fast Technique to Estimate Nuclear Genome Dimension of Zygnematophycean Inexperienced Algae

Zygnematophyceae inexperienced algae (ZGA) have been proven to be the closest relations of land crops. Three nuclear genomes of ZGA have been not too long ago printed, and extra genomes are underway. Right here we analyzed two Zygnema circumcarinatum strains SAG 698-1a (mating +) and SAG 698-1b (mating -) and located distinct cell sizes and different morphological variations. The molecular identities of the 2 strains had been additional investigated by sequencing their 18S rRNA, psaA and rbcL genes. These marker genes of SAG 698-1a had been surprisingly far more just like Z. cylindricum (SAG 698-2) than to SAG 698-1b.

Phylogenies of those marker genes additionally confirmed that SAG 698-1a and SAG 698-1b had been effectively separated into two completely different Zygnema clades, the place SAG 698-1a was clustered with Z. cylindricum, whereas SAG 698-1b was clustered with Z. tunetanum. Moreover, physiological parameters like ETRmax values differed between SAG 698-1a and SAG 698-1b after 2 months of cultivation. The de-epoxidation state (DEPS) of the xanthophyll cycle pigments additionally confirmed vital variations. Surprisingly, the 2 strains couldn’t conjugate, and considerably differed within the thickness of the mucilage layer.

Recombinant Humanp21 Recombinant Protein
92-035
Recombinant phosphopantetheine adenylyltransferase (PPAT) (Recombinant)
20-abx072018
Glyco Recombinant Protein A33 Recombinant Protein
96-361
Glyco Recombinant Protein A33 Recombinant Protein
96-364
STAG3L1 Recombinant Protein (Human) (Recombinant Tag)
RP030283

Moreover, ZGA cell partitions are extremely enriched with sticky and acidic polysaccharides, and subsequently the extensively used plant nuclear extraction protocols don’t work effectively in ZGA. Right here, we additionally report a quick and easy technique, by mechanical chopping, for environment friendly nuclear extraction within the two SAG strains. Extra importantly, the extracted nuclei had been additional used for nuclear genome dimension estimation of the 2 SAG strains by move cytometry (FC).

Morpheus HT-96 Green Screen

M-MD1-47-GREEN 96 x 1 ml ml
EUR 280
Description: Morpheus HT-96 Green Screen

Antibody for Algae DNAJ

SPC-300B 0.2ml
EUR 439.2
Description: A polyclonal antibody for DNAJ from Algae | Algae (Chlamydomonas reinhardtii) | Bacteria | E. coli (Escherichia coli). The antibody is produced in rabbit after immunization with Algae Recombinant DNAJ of Chlamydomonas reinhardtii. The Antibody is tested and validated for WB, AfP assays with the following recommended dilutions: WB (1:5000). This DNAJ antibody is unconjugated.

Antibody for Algae DNAK

SPC-301B 0.2ml
EUR 439.2
Description: A polyclonal antibody for DNAK from Algae | Algae (Chlamydomonas reinhardtii). The antibody is produced in rabbit after immunization with Algae Recombinant DNAK of Chlamydomonoas reinhardtii. The Antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:5000). This DNAK antibody is unconjugated.

Antibody for Algae HSP70B (Plant)

SPC-315D 0.1ml
EUR 439.2
Description: A polyclonal antibody for HSP70 from Algae | Plant | Algae (Chlamydomonas reinhardtii) | Algae (Desmodesmus subspicatus) | Moss (Physcomitrella patens). The antibody is produced in rabbit after immunization with Algae Mature HSP70B protein expressed with N- and C- terminal hexahistidine tags in E.coli, purified with Ni-NTA. The Antibody is tested and validated for WB, IP assays with the following recommended dilutions: WB (1:10000). This HSP70 antibody is unconjugated.

Antibody for Algae HSP90C (Plant)

SPC-317D 0.1ml
EUR 439.2
Description: A polyclonal antibody for HSP90 from Algae | Algae (Chlamydomonas reinhardtii). The antibody is produced in rabbit after immunization with Algae C-terminal 238 amino acids of HSP90C expressed with N- and C-terminal hexahistidine tags in E.coli, purified with Ni-NTA.. The Antibody is tested and validated for WB, IP assays with the following recommended dilutions: WB (1:3000). This HSP90 antibody is unconjugated.

Cassettes in QuickLoad Sleeves

M480-4SL-GREEN 1 ea.
EUR 340.8

Thiazole Green, 10, 000X in DMSO

40086-0.5mL 0.5mL
EUR 225.6
Description: Minimum order quantity: 1 unit of 0.5mL

Thiazole Green, 10, 000X in DMSO

40086-1mL 1mL
EUR 344.4
Description: Minimum order quantity: 1 unit of 1mL

MitoView Green

70054 20X50UG
EUR 271.2
Description: Minimum order quantity: 1 unit of 20X50UG

Bromocresol green

20-abx186394
  • EUR 493.20
  • EUR 292.80
  • 100 g
  • 25 g

Malachite green

AG060 1 mg
EUR 627.6

Leucomalachite green

AG105 1 mg
EUR 627.6

Brilliant green

BB0242 25g
EUR 67.31

Malachite green

AT060 1mg
EUR 2148

Leucomalachite green

AT105 1mg
EUR 1641.6

Bromocresol Green

GT4023-25G 25 g
EUR 132

Bromocresol Green

GT4023-5G 5 g
EUR 64.8

Leucomalachite Green

GT7606-10G 10 g
EUR 112.8

Leucomalachite Green

GT7606-25G 25 g
EUR 189.6

Leucomalachite Green

GT7606-5G 5 g
EUR 79.2

Insulin (human) recombinant expressed in yeast

B7407-10 10 mg
EUR 174
Description: IC50: 5.01 nmol/L for insulin receptorInsulin is a peptide hormone produced by beta cells in pancreas. Insulin has been found to stimulate glucose uptake into muscle and adipose tissue, which is central to the maintenance of whole body glucose homeostasis.

Ribonuclease H, expressed in E. coli

GZ5072-1KU 1 KU
EUR 600

Ribonuclease H, expressed in E. coli

GZ5072-250U 250 U
EUR 255.6

Reverse Transcriptase, expressed in E. coli

GZ8080-10KIU 10 KIU
EUR 342

Reverse Transcriptase, expressed in E. coli

GZ8080-25KIU 25 KIU
EUR 648

Screw Cap In w O-ring - Green

TUB1126 PK3000
EUR 121.98

PhosLite™ Green

11630 1 mg
EUR 262.8

Malachite green Antibody

48363-100ul 100ul
EUR 399.6

Malachite green Antibody

48363-50ul 50ul
EUR 286.8

BactoViewâ„¢ Live Green

40102 100uL
EUR 384
Description: Minimum order quantity: 1 unit of 100uL

Malachite Green antibody

20-1184 100 ul
EUR 1054.8
Description: Sheep polyclonal Malachite Green antibody

Thiolite™ Green

21508 5 mg
EUR 367.2

CytoTell™ Green

22253 500 Tests
EUR 158.4

CytoTell™ Green

22254 1000 Tests
EUR 211.2

LysoBrite™ Green

22643 500 Tests
EUR 211.2

ViaVac Red/Green

29068 100uL
EUR 313.2
Description: Minimum order quantity: 1 unit of 100uL

Fast Green FCF

abx082188-5g 5 g
EUR 243.6

Green qPCR SuperMix

20-abx098031
  • EUR 1245.60
  • EUR 526.80
  • EUR 760.80
  • 15 ml
  • 1 ml
  • 5 ml

Malachite green oxalate

abx180007-25g 25 g
EUR 326.4

C.I.Solvent Green 3

abx185369-25g 25 g
EUR 610.8

Leucomalachite green-HRP

80-1192 500 ul
EUR 164.4
Description: Leucomalachite green Conjugate for use in immunoassays

ER-Hunt Green

9548-100
EUR 535.2

Leuco- Malachiet green

AG131 1 mg
EUR 627.6

BRILLIANT GREEN AGAR

B02-115-10kg 10 kg
EUR 1466.4

BRILLIANT GREEN AGAR

B02-115-2Kg 2 Kg
EUR 366

BRILLIANT GREEN AGAR

B02-115-500g 500 g
EUR 142.8

SYBR Green I

B1747-25
EUR 1396.8

SYBR Green I

B1747-5
EUR 464.4

Green Fluorescent Protein

CH22124 100 ul of 1 mg/ml
EUR 522

Malachite Green [HRP]

DAGA-073H 1mg
EUR 1404

Leucomalachite Green [HRP]

DAGA-074H 1mg
EUR 1326

Malachite Green [BSA]

DAGA-085B 1mg
EUR 936

Leucomalachite Green [BSA]

DAGA-086B 1mg
EUR 936

Malachite Green [KLH]

DAGA-091K 1mg
EUR 1326

Leucomalachite Green [KLH]

DAGA-092K 1mg
EUR 1170

DNASE & METHYL GREEN

D04-102-10kg 10 kg
EUR 11270.4

DNASE & METHYL GREEN

D04-102-2kg 2kg
EUR 2497.2

DNASE & METHYL GREEN

D04-102-500g 500 g
EUR 722.4

0.6ML MICROTUBES. GREEN

MCT-060-G 1000/pk
EUR 219.6
Description: Micro Tubes; Micro Tubes Push Cap- Axygen

1.7ML MICROTUBES. GREEN

MCT-175-G 500/pk
EUR 139.2
Description: Micro Tubes; Micro Tubes Push Cap- Axygen

Malachite green oxalate

MD0330 50g
EUR 69.4

Green Fluorescent Protein

MO22184 100 ul
EUR 490.8

Green Taq Mix

P131-01 5 ml
EUR 142.8

Green Taq Mix

P131-02 15 ml
EUR 180

Green Taq Mix

P131-03 50 ml
EUR 297.6

Indocyanine green(Cardiogreen)

E1KS1924 50 mg
EUR 296.4

Light Green Solution

LGA030 30 ml
EUR 70.8

Light Green Solution

LGA125 125 ml
EUR 79.2

Light Green Solution

LGA3800 1 Gal.
EUR 325.2

Light Green Solution

LGA500 500 ml
EUR 121.2

Light Green Solution

LGA999 1000 ml
EUR 168

Safe-Green™

G108-G 1.0 ml
EUR 85.2

Fast Green FCF

HY-D0914 500mg
EUR 129.6

Fast Green FCF

FB0452 5g
EUR 66.26

Fast Green Solution

FGN125 125 ml
EUR 79.2

Fast Green Solution

FGN500 500 ml
EUR 115.2

Fast Green Solution

FGN999 1000 ml
EUR 148.8

Acid Green 50

GT1171-100G 100 g
EUR 103.2

Acid Green 50

GT1171-25G 25 g
EUR 55.2

Acid Green 50

GT1171-500G 500 g
EUR 274.8

Reactive Green 5

GT6783-25G 25 g
EUR 151.2

Reactive Green 5

GT6783-5G 5 g
EUR 64.8

Accuris qMax Green

PR2000-H-100 1 PC
EUR 138.29

Accuris qMax Green

PR2000-H-1000 1 PC
EUR 561.29

Accuris qMax Green

PR2000-H-500 1 PC
EUR 341.17

Accuris qMax Green

PR2000-H-S 1 PC
EUR 88.52

Accuris qMax Green

PR2000-L-100 1 PC
EUR 138.29

Accuris qMax Green

PR2000-L-1000 1 PC
EUR 561.29

Accuris qMax Green

PR2000-L-500 1 PC
EUR 341.17

Accuris qMax Green

PR2000-L-S 1 PC
EUR 88.52

Accuris qMax Green

PR2000-N-100 1 PC
EUR 136.38

Accuris qMax Green

PR2000-N-1000 1 PC
EUR 551.71

Accuris qMax Green

PR2000-N-500 1 PC
EUR 335.44

Accuris qMax Green

PR2000-N-S 1 PC
EUR 87.58

ViSafe Green Gel

S435 500 µl
EUR 175.2

ViSafe Green Gel

S435L 5x500 µl
EUR 668.4

Green-DNA Dye

SB2040 100UL, 100UL
EUR 80.88

Cap Green Schott

29338286 PK10
EUR 21.26

CoolBox XT green

432022 EACH
EUR 622.44

CoolRack M6 green.

432035 EACH
EUR 80.94

CoolRack M15 green

432038 EACH
EUR 188.1

CoolRack M30 green

432042 EACH
EUR 327.18

Pouring Ring Green

1089911 PK10
EUR 7.98

Hinged CryoBox green

ICE4174 PK5
EUR 68.4

Brilliant Green Agar

MED1372 EACH
EUR 69.54

Simport Capinsert Green

MIC2706 PK500
EUR 23.71

Tough 13mm Green

MIC5016 PK1000
EUR 55.97

5G Bromocresol Green

NAT1356 5G
EUR 144.78

10G Methyl Green

NAT1364 10G
EUR 172.14

Capinsert 5.3mm Green

CRY2064 PK500
EUR 40.36

Parafilm dispenser green

DD320049 EACH
EUR 117.42

Simport Slidefolder Green

HIS0253 PK10
EUR 160.74

Simport Slidefile Green

HIS0259 PK10
EUR 160.74

Simport Slidemailer Green

HIS0269 PK200
EUR 74.1

ScrewCap Green CentreHole

CHR3368 PK1000
EUR 484.5

Protect beads green

SWA1151 PK80
EUR 103.74

Innoculation loop green

TS/2-B PK1000
EUR 92.34

Screw Cap - Green

TUB1140 PK3000
EUR 128.82

Simport Unirack Green

RAC2102 PK10
EUR 49.7

JBS Xtal Green

M-CO-303 300 µl
EUR 15
Description: JBS Xtal Green

BS8599-1 Large Workplace First Aid Kit - in Large Integral Aura Box Green/Green

SAF1188 EACH
EUR 42.75

BS8599-1 Medium Workplace First Aid Kit - in Medium Integral Aura Box Green/Green

SAF1272 EACH
EUR 33.4

BS8599-1 Small Workplace First Aid Kit - in Small Integral Aura Box Green/Green

SAF1278 EACH
EUR 50.27

Embedding Cassettes

M480-4-GREEN 1 ea.
EUR 232.8

Preferentially Expressed Antigen In Melanoma (PRAME) Antibody

20-abx104373
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Preferentially Expressed Antigen In Melanoma (PRAME) Antibody

20-abx104374
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Melanoma antigen preferentially expressed in tumors Antibody

20-abx109324
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Highly Expressed In Cancer Protein (HEC1) Antibody

abx432790-200ul 200 ul
EUR 460.8

rec Human Growth Hormone (expressed in E.coli)

H-3148.0500 0.5mg
EUR 1096.8

rec Human Growth Hormone (expressed in E.coli)

H-3148.1000 1.0mg
EUR 1936.8

T4 DNA Ligase, expressed in E. coli

GZ9900-1KIU 1 KIU
EUR 217.2

T4 DNA Ligase, expressed in E. coli

GZ9900-5KIU 5 KIU
EUR 562.8

Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)

4-RPH323Hu01
  • EUR 631.80
  • EUR 292.80
  • EUR 2039.23
  • EUR 759.74
  • EUR 1399.49
  • EUR 498.00
  • EUR 4918.08
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Preferentially Expressed Antigen In Melanoma expressed in: E.coli

Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)

4-RPH323Hu02
  • EUR 631.80
  • EUR 292.80
  • EUR 2039.23
  • EUR 759.74
  • EUR 1399.49
  • EUR 498.00
  • EUR 4918.08
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Preferentially Expressed Antigen In Melanoma expressed in: E.coli

Bromocresol Green Indicator 0.1% (w/v) in IPA

BRCGIPA0105 500ML
EUR 69.54

Bromocresol Green Indicator 0.04% (w/v) in MeOH

BRCGM05 500ML
EUR 60.42

Human Preferentially Expressed Antigen In Melanoma (PRAME) Protein

20-abx068652
  • EUR 878.40
  • EUR 343.20
  • EUR 2749.20
  • EUR 1045.20
  • EUR 627.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Preferentially Expressed Antigen In Melanoma (PRAME) Protein

20-abx068653
  • EUR 878.40
  • EUR 343.20
  • EUR 2749.20
  • EUR 1045.20
  • EUR 627.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Melanoma Antigen Preferentially Expressed In Tumors (PRAME) Antibody

20-abx004948
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Differentially Expressed In FDCP 6 Homolog (DEF6) Antibody

20-abx112066
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Amy1 Associated Protein Expressed In Testis (AAT1) Antibody

20-abx129058
  • EUR 543.60
  • EUR 159.60
  • EUR 1562.40
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Non-Metastatic Cells 3, Protein Expressed In Antibody

20-abx114145
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

To verify the FC end result, we’ve got additionally used different experimental strategies for nuclear genome dimension estimation of the 2 strains. Curiously, the 2 strains had been discovered to have very distinct nuclear genome sizes. Our a number of traces of proof strongly point out that SAG 698-1a presumably had been confused with SAG 698-2 previous to 2005, and almost definitely represents Z. cylindricum or a intently associated species.

ASAP1 antibody Antibody
DF8746
CD11b Antibody Antibody
ABD2911
anti- Antibody^Polyclonal antibody control antibody
LSMab09882
Antibody
A1360-500
Ly1 Antibody Reactive (LYAR) Antibody
20-abx123734

 

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