Chromochloris zofingiensis (Chlorophyceae) Divides by Consecutive Multiple Fission Cell-Cycle under Batch and Continuous Cultivation

Chromochloris zofingiensis (Chlorophyceae) Divides by Consecutive Multiple Fission Cell-Cycle under Batch and Continuous Cultivation

A number of inexperienced algae can divide by a number of fission and spontaneously synchronize their cell cycle with the obtainable mild regime. The yields that may be obtained from a microalgal tradition are instantly affected by cell cycle occasions. Chromochloris zofingiensis is taken into account as one of the crucial promising microalgae for biotechnological purposes because of its quick progress and the versatile trophic capabilities. It’s intensively investigated within the context of bio-commodities manufacturing (carotenoids, storage lipids); nonetheless, the sample of cell-cycle occasions underneath frequent cultivation methods was not but characterised for C. zofingiensis.

On this examine, we’ve got employed fluorescence microscopy to characterize the fundamental cell-cycle dynamics underneath batch and steady modes of phototrophic C. zofingiensis cultivation. Staining with SYBR green-applied in DMSO solution-enabled, for the primary time, the clear and easy visualization of polynuclear phases on this microalga. Accordingly, we concluded that C. zofingiensis divides by a consecutive sample of a number of fission, whereby it spontaneously synchronizes progress and cell division in keeping with the obtainable illumination regime.

In high-light steady tradition or low-light batch tradition, C. zofingiensis cell-cycle was accomplished inside a number of light-dark (L/D) cycles (14 h/10 h); nonetheless, cell divisions had been synchronized with the darkish intervals solely within the high-light steady tradition. In each modes of cultivation, daughter cell launch was primarily facilitated by division of eight and 16-polynuclear cells. The outcomes of this examine are of each elementary and utilized science significance and are additionally necessary for the event of an environment friendly nuclear transformation system for C. zofingiensis.

Exploration of making use of growth-promotion micro organism of Chlorella sorokiniana to open cultivation programs

These days, synthetic development of bacteria-algae consortia to reinforce microalgal biomass is prevalent in enclosed programs, whereas few are in-built an open tradition. On this examine, Achromobacter sp. and Rhizobium sp., remoted from an open pond of Chlorella sorokiniana, had been the microalgal growth-promotion micro organism and chosen to construct the bacteria-algae consortia with axenic C. sorokiniana in open cultivation programs. To look at the efficiency of those two synthetic bacteria-algae consortia in open tradition underneath steady cultivation circumstances, the co-cultivation experiments had been carried out underneath fixed temperature and lightweight depth indoors.

It was discovered that Achromobacter sp. progressively misplaced the dominance of the inhabitants within the co-culture and failed to advertise the expansion of C. sorokiniana throughout open cultivation. Nonetheless, the Rhizobium sp. maintained its dominant inhabitants of bacterial neighborhood in open tradition and will promote the expansion of C. sorokiniana, with an enhancement of 13.76%. To additional consider the results of Rhizobium sp. on microalgae underneath variations of temperature and daylight depth circumstances, the open co-cultivation experiments had been constructed outdoor.

Outcomes confirmed that the expansion of C. sorokiniana may rise 13.29% solely when Rhizobium sp. was added to the tradition repeatedly, and addition of bacterial resolution in log-phase of microalgae may assist Rhizobium sp. dominate within the bacterial neighborhood. On this approach, addition of Rhizobium sp. within the log-phase of C. sorokiniana needs to be an efficient course of to be utilized to open ponds cultivation. Our findings are a step towards making use of growth-promotion micro organism for C. sorokiniana for purposes in open cultivation programs.

Chromochloris zofingiensis (Chlorophyceae) Divides by Consecutive Multiple Fission Cell-Cycle under Batch and Continuous Cultivation

Characterization of Two Zygnema Strains ( Zygnema circumcarinatum SAG 698-1a and SAG 698-1b) and a Fast Technique to Estimate Nuclear Genome Dimension of Zygnematophycean Inexperienced Algae

Zygnematophyceae inexperienced algae (ZGA) have been proven to be the closest relations of land crops. Three nuclear genomes of ZGA have been not too long ago printed, and extra genomes are underway. Right here we analyzed two Zygnema circumcarinatum strains SAG 698-1a (mating +) and SAG 698-1b (mating -) and located distinct cell sizes and different morphological variations. The molecular identities of the 2 strains had been additional investigated by sequencing their 18S rRNA, psaA and rbcL genes. These marker genes of SAG 698-1a had been surprisingly far more just like Z. cylindricum (SAG 698-2) than to SAG 698-1b.

Phylogenies of those marker genes additionally confirmed that SAG 698-1a and SAG 698-1b had been effectively separated into two completely different Zygnema clades, the place SAG 698-1a was clustered with Z. cylindricum, whereas SAG 698-1b was clustered with Z. tunetanum. Moreover, physiological parameters like ETRmax values differed between SAG 698-1a and SAG 698-1b after 2 months of cultivation. The de-epoxidation state (DEPS) of the xanthophyll cycle pigments additionally confirmed vital variations. Surprisingly, the 2 strains couldn’t conjugate, and considerably differed within the thickness of the mucilage layer.

Moreover, ZGA cell partitions are extremely enriched with sticky and acidic polysaccharides, and subsequently the extensively used plant nuclear extraction protocols don’t work effectively in ZGA. Right here, we additionally report a quick and easy technique, by mechanical chopping, for environment friendly nuclear extraction within the two SAG strains. Extra importantly, the extracted nuclei had been additional used for nuclear genome dimension estimation of the 2 SAG strains by move cytometry (FC).

OPTIONAL GREEN FILTER (572NM)

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EUR 1052
Description: Lab Equipment; Axygen Branded EQ

Antibody for Algae DNAJ

SPC-300B 0.2ml
EUR 366
  • DNAJ is a prokaryotic heat shock induced protein from the bacterium E.coli, which is under control of the htpr regulatory protein (1). DNAJ interacts with the chaperone HSP70-like DNAK protein to disassemble a protein complex found at a phage lambda'
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Description: A polyclonal antibody for DNAJ from Algae | Algae (Chlamydomonas reinhardtii) | Bacteria | E. coli (Escherichia coli). The antibody is produced in rabbit after immunization with Algae Recombinant DNAJ of Chlamydomonas reinhardtii. The Antibody is tested and validated for WB, AfP assays with the following recommended dilutions: WB (1:5000). This DNAJ antibody is unconjugated.

Antibody for Algae DNAK

SPC-301B 0.2ml
EUR 366
  • The prokaryotic HSP70 protein family includes DNA K, HSCA (HSC66), and HSCC (HSC62) (1). DNAK interacts with DNAJ and GrpE heat shock protein chaperones to fold substrate proteins in an ATP-driven cycle (2). These proteins are also involved in cell p
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Description: A polyclonal antibody for DNAK from Algae | Algae (Chlamydomonas reinhardtii). The antibody is produced in rabbit after immunization with Algae Recombinant DNAK of Chlamydomonoas reinhardtii. The Antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:5000). This DNAK antibody is unconjugated.

Antibody for Algae HSP70B (Plant)

SPC-315D 0.1ml
EUR 366
  • HSP70B (heat shock protein 70B) is a nuclear-encoded, chloroplast-targeted chaperone of the HSP70 family. It is the major HSP70 in the stroma of Chlamydomonas chloroplasts. It interacts with HSP90C, CGE1, CDJ2 and VIPP1 (1-3). It has been shown that
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Description: A polyclonal antibody for HSP70 from Algae | Plant | Algae (Chlamydomonas reinhardtii) | Algae (Desmodesmus subspicatus) | Moss (Physcomitrella patens). The antibody is produced in rabbit after immunization with Algae Mature HSP70B protein expressed with N- and C- terminal hexahistidine tags in E.coli, purified with Ni-NTA. The Antibody is tested and validated for WB, IP assays with the following recommended dilutions: WB (1:10000). This HSP70 antibody is unconjugated.

Antibody for Algae HSP90C (Plant)

SPC-317D 0.1ml
EUR 366
  • HSP90C is a nuclear-encoded, chloroplast-targeted chaperone of the HSP90 family. In the chloroplast, it is localized to the soluble stroma fraction, but also to thylakoids and low-density membranes containing inner envelopes. HSP90C is expressed unde
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Description: A polyclonal antibody for HSP90 from Algae | Algae (Chlamydomonas reinhardtii). The antibody is produced in rabbit after immunization with Algae C-terminal 238 amino acids of HSP90C expressed with N- and C-terminal hexahistidine tags in E.coli, purified with Ni-NTA.. The Antibody is tested and validated for WB, IP assays with the following recommended dilutions: WB (1:3000). This HSP90 antibody is unconjugated.

Cassettes in QuickLoad Sleeves

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MitoView Green

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Malachite green

AT060 1mg
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Leucomalachite green

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20-abx186394
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GT4023-5G 5 g
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GT7606-10G 10 g
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  • Product category: Biochemicals/Indicators/Stains/Biological Stains

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29068 100uL
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22253 500 Tests
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22254 1000 Tests
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22643 500 Tests
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21508 5 mg
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48363-100ul 100ul
EUR 333

Malachite green Antibody

48363-50ul 50ul
EUR 239

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20-1184 100 ul
EUR 879
Description: Sheep polyclonal Malachite Green antibody

PhosLite™ Green

11630 1 mg
EUR 219
  • R-phrase: R20, R21, R22
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  • UNSPEC Code: 12171501

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40102 100uL
EUR 320
Description: Minimum order quantity: 1 unit of 100uL

SYBR Green I

B1747-25
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B1747-5
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DAGA-073H 1mg
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DAGA-074H 1mg
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EUR 780

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Leucomalachite green-HRP

80-1192 500 ul
EUR 137
Description: Leucomalachite green Conjugate for use in immunoassays

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E1KS1924 50 mg
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CH22124 100 ul of 1 mg/ml
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Leuco- Malachiet green

AG131 1 mg
EUR 523

Fast Green FCF

HY-D0914 500mg
EUR 108

Reactive Green 5

GT6783-25G 25 g
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Reactive Green 5

GT6783-5G 5 g
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Safe-Green™

G108-G 1.0 ml
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GT1171-100G 100 g
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GT1171-500G 500 g
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FGN999 1000 ml
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P131-02 15 ml
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P131-03 50 ml
EUR 248

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LGA030 30 ml
EUR 59

Light Green Solution

LGA125 125 ml
EUR 66

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LGA3800 1 Gal.
EUR 271

Light Green Solution

LGA500 500 ml
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Light Green Solution

LGA999 1000 ml
EUR 140

5G Bromocresol Green

NAT1356 5G
EUR 156

10G Methyl Green

NAT1364 10G
EUR 176

Green Fluorescent Protein

MO22184 100 ul
EUR 409

0.6ML MICROTUBES. GREEN

MCT-060-G 1000/pk
EUR 183
Description: Micro Tubes; Micro Tubes Push Cap- Axygen

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MCT-175-G 500/pk
EUR 116
Description: Micro Tubes; Micro Tubes Push Cap- Axygen

Accuris qMax Green

PR2000-H-100 1 PC
EUR 115.24
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PR2000-H-1000 1 PC
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PR2000-L-100 1 PC
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Accuris qMax Green

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Accuris qMax Green

PR2000-L-500 1 PC
EUR 284.31
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Accuris qMax Green

PR2000-L-S 1 PC
EUR 73.77
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PR2000-N-100 1 PC
EUR 113.65
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Accuris qMax Green

PR2000-N-1000 1 PC
EUR 459.76
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Accuris qMax Green

PR2000-N-500 1 PC
EUR 279.53
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Accuris qMax Green

PR2000-N-S 1 PC
EUR 72.98
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ViSafe Green Gel

S435 500 µl
EUR 146

ViSafe Green Gel

S435L 5x500 µl
EUR 557

Fast Green FCF

FB0452 5g
EUR 55.22
  • Product category: Biochemicals/Indicators/Stains/Peptide/Protein Related

Malachite green oxalate

MD0330 50g
EUR 57.83
  • Product category: Biochemicals/Indicators/Stains/Biological Stains

Green-DNA Dye

SB2040 100UL, 100UL
EUR 67.4
  • Product category: Electrophoresis Related/Tracking Dyes

Insulin (human) recombinant expressed in yeast

B7407-10 10 mg
EUR 145
Description: IC50: 5.01 nmol/L for insulin receptorInsulin is a peptide hormone produced by beta cells in pancreas. Insulin has been found to stimulate glucose uptake into muscle and adipose tissue, which is central to the maintenance of whole body glucose homeostasis.

Ribonuclease H, expressed in E. coli

GZ5072-1KU 1 KU
EUR 500

Ribonuclease H, expressed in E. coli

GZ5072-250U 250 U
EUR 213

Reverse Transcriptase, expressed in E. coli

GZ8080-10KIU 10 KIU
EUR 285

Reverse Transcriptase, expressed in E. coli

GZ8080-25KIU 25 KIU
EUR 540

MitoLite™ Green EX488

22675 500 Tests
EUR 132
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

MitoLite™ Green FM

22695 10x50 ug
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

MycoLight™ Green JJ98

24000 100 ul
EUR 219
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

MycoLight™ Green JJ99

24001 100 ul
EUR 219
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

Protonex™ Green 500

21215 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Recombinant Green Fluorescent Protein

7-06112 10µg Ask for price

Recombinant Green Fluorescent Protein

7-06113 50µg Ask for price

Recombinant Green Fluorescent Protein

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MitoView Green, Trial Size

70054-T 50UG
EUR 94
Description: Minimum order quantity: 1 unit of 50UG

MMP Green™ substrate

13520 1 mg
EUR 480
  • R-phrase: R20, R21, R22
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  • UNSPEC Code: 12352200

Nuclear Green™ LCS1

17540 0.5 ml
EUR 132
  • R-phrase: R20, R21, R68
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  • UNSPEC Code: 41116134

Nuclear Green™ DCS1

17550 0.5 ml
EUR 132
  • R-phrase: R20, R21, R68
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  • UNSPEC Code: 41116134

EZSolution? SYBR Green I

B1748-1
EUR 620

EZSolution? SYBR Green I

B1748-500
EUR 430

BRILLIANT GREEN BILE AGAR

B02-122-10kg 10 kg
EUR 1267

BRILLIANT GREEN BILE AGAR

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EUR 315

BRILLIANT GREEN BILE AGAR

B02-122-500g 500 g
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Malachite Green ELISA Kit

DEIA057 96T
EUR 1251
Description: The Malachite Green ELISA Kit is a direct competitive enzyme immunoassay for the quantitative analysis of Malachite green in fish and shrimp samples.

Green qPCR SuperMix UDG

20-abx098032
  • EUR 1135.00
  • EUR 453.00
  • EUR 662.00
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Top Green qPCR SuperMix

20-abx098033
  • EUR 829.00
  • EUR 217.00
  • EUR 1135.00
  • EUR 411.00
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Tip Green qPCR SuperMix

20-abx098034
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  • EUR 1330.00
  • EUR 467.00
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Green Fluorescent Protein (GFP)

CH23105 400 ul
EUR 761

Malachite green Conjugated Antibody

C48363 100ul
EUR 397

Bromocresol Green sodium salt

GT3714-100G 100 g
EUR 229

Bromocresol Green sodium salt

GT3714-25G 25 g
EUR 110

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GT3714-5G 5 g
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Brilliant Green (C.I. 42040)

GT6123-100G 100 g
EUR 78

Brilliant Green (C.I. 42040)

GT6123-25G 25 g
EUR 46

Malachite green carbinol base

GT7477-100G 100 g
EUR 96

Methyl Green (C.I. 42585)

GT7495-100G 100 g
EUR 198

Methyl Green (C.I. 42585)

GT7495-25G 25 g
EUR 94

Methyl Green (C.I. 42585)

GT7495-5G 5 g
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Malachite Green carbinol hydrochloride

GT8088-100G 100 g
EUR 114

Malachite Green carbinol hydrochloride

GT8088-25G 25 g
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DNA Loading Green Dye

G924 6X; 3 x 1.0 ml
EUR 79

Green Lysis Kit 50

GREENE1 50pack
EUR 156
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads.

Green Lysis Kit 250

GREENE5 250pack
EUR 468
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Polycaspase Assay Kit, green

KF17200 25 Tests
EUR 304

Novel Green PLUS (20000X)

LD003-0500 500 µl
EUR 172

Novel Green PLUS (20000X)

LD003-0500L 5x500 µl
EUR 731

Light Green Solution (0.1%)

LGD-10000 10 L
EUR 886

Light Green Solution (0.1%)

LGD125 125 ml
EUR 72

Light Green Solution (0.1%)

LGD3800 1 Gal.
EUR 397

Light Green Solution (0.1%)

LGD500 500 ml
EUR 101

To verify the FC end result, we’ve got additionally used different experimental strategies for nuclear genome dimension estimation of the 2 strains. Curiously, the 2 strains had been discovered to have very distinct nuclear genome sizes. Our a number of traces of proof strongly point out that SAG 698-1a presumably had been confused with SAG 698-2 previous to 2005, and almost definitely represents Z. cylindricum or a intently associated species.

 

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