A number of inexperienced algae can divide by a number of fission and spontaneously synchronize their cell cycle with the obtainable mild regime. The yields that may be obtained from a microalgal tradition are instantly affected by cell cycle occasions. Chromochloris zofingiensis is taken into account as one of the crucial promising microalgae for biotechnological purposes because of its quick progress and the versatile trophic capabilities. It’s intensively investigated within the context of bio-commodities manufacturing (carotenoids, storage lipids); nonetheless, the sample of cell-cycle occasions underneath frequent cultivation methods was not but characterised for C. zofingiensis.
On this examine, we’ve got employed fluorescence microscopy to characterize the fundamental cell-cycle dynamics underneath batch and steady modes of phototrophic C. zofingiensis cultivation. Staining with SYBR green-applied in DMSO solution-enabled, for the primary time, the clear and easy visualization of polynuclear phases on this microalga. Accordingly, we concluded that C. zofingiensis divides by a consecutive sample of a number of fission, whereby it spontaneously synchronizes progress and cell division in keeping with the obtainable illumination regime.
In high-light steady tradition or low-light batch tradition, C. zofingiensis cell-cycle was accomplished inside a number of light-dark (L/D) cycles (14 h/10 h); nonetheless, cell divisions had been synchronized with the darkish intervals solely within the high-light steady tradition. In each modes of cultivation, daughter cell launch was primarily facilitated by division of eight and 16-polynuclear cells. The outcomes of this examine are of each elementary and utilized science significance and are additionally necessary for the event of an environment friendly nuclear transformation system for C. zofingiensis.
Exploration of making use of growth-promotion micro organism of Chlorella sorokiniana to open cultivation programs
These days, synthetic development of bacteria-algae consortia to reinforce microalgal biomass is prevalent in enclosed programs, whereas few are in-built an open tradition. On this examine, Achromobacter sp. and Rhizobium sp., remoted from an open pond of Chlorella sorokiniana, had been the microalgal growth-promotion micro organism and chosen to construct the bacteria-algae consortia with axenic C. sorokiniana in open cultivation programs. To look at the efficiency of those two synthetic bacteria-algae consortia in open tradition underneath steady cultivation circumstances, the co-cultivation experiments had been carried out underneath fixed temperature and lightweight depth indoors.
It was discovered that Achromobacter sp. progressively misplaced the dominance of the inhabitants within the co-culture and failed to advertise the expansion of C. sorokiniana throughout open cultivation. Nonetheless, the Rhizobium sp. maintained its dominant inhabitants of bacterial neighborhood in open tradition and will promote the expansion of C. sorokiniana, with an enhancement of 13.76%. To additional consider the results of Rhizobium sp. on microalgae underneath variations of temperature and daylight depth circumstances, the open co-cultivation experiments had been constructed outdoor.
Outcomes confirmed that the expansion of C. sorokiniana may rise 13.29% solely when Rhizobium sp. was added to the tradition repeatedly, and addition of bacterial resolution in log-phase of microalgae may assist Rhizobium sp. dominate within the bacterial neighborhood. On this approach, addition of Rhizobium sp. within the log-phase of C. sorokiniana needs to be an efficient course of to be utilized to open ponds cultivation. Our findings are a step towards making use of growth-promotion micro organism for C. sorokiniana for purposes in open cultivation programs.
Characterization of Two Zygnema Strains ( Zygnema circumcarinatum SAG 698-1a and SAG 698-1b) and a Fast Technique to Estimate Nuclear Genome Dimension of Zygnematophycean Inexperienced Algae
Zygnematophyceae inexperienced algae (ZGA) have been proven to be the closest relations of land crops. Three nuclear genomes of ZGA have been not too long ago printed, and extra genomes are underway. Right here we analyzed two Zygnema circumcarinatum strains SAG 698-1a (mating +) and SAG 698-1b (mating -) and located distinct cell sizes and different morphological variations. The molecular identities of the 2 strains had been additional investigated by sequencing their 18S rRNA, psaA and rbcL genes. These marker genes of SAG 698-1a had been surprisingly far more just like Z. cylindricum (SAG 698-2) than to SAG 698-1b.
Phylogenies of those marker genes additionally confirmed that SAG 698-1a and SAG 698-1b had been effectively separated into two completely different Zygnema clades, the place SAG 698-1a was clustered with Z. cylindricum, whereas SAG 698-1b was clustered with Z. tunetanum. Moreover, physiological parameters like ETRmax values differed between SAG 698-1a and SAG 698-1b after 2 months of cultivation. The de-epoxidation state (DEPS) of the xanthophyll cycle pigments additionally confirmed vital variations. Surprisingly, the 2 strains couldn’t conjugate, and considerably differed within the thickness of the mucilage layer.
Moreover, ZGA cell partitions are extremely enriched with sticky and acidic polysaccharides, and subsequently the extensively used plant nuclear extraction protocols don’t work effectively in ZGA. Right here, we additionally report a quick and easy technique, by mechanical chopping, for environment friendly nuclear extraction within the two SAG strains. Extra importantly, the extracted nuclei had been additional used for nuclear genome dimension estimation of the 2 SAG strains by move cytometry (FC).
Antibody for Algae DNAJ |
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| SPC-300B | Stressmarq | 0.2ml | EUR 366 |
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Description: A polyclonal antibody for DNAJ from Algae | Algae (Chlamydomonas reinhardtii) | Bacteria | E. coli (Escherichia coli). The antibody is produced in rabbit after immunization with Algae Recombinant DNAJ of Chlamydomonas reinhardtii. The Antibody is tested and validated for WB, AfP assays with the following recommended dilutions: WB (1:5000). This DNAJ antibody is unconjugated. |
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Antibody for Algae DNAK |
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| SPC-301B | Stressmarq | 0.2ml | EUR 366 |
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Description: A polyclonal antibody for DNAK from Algae | Algae (Chlamydomonas reinhardtii). The antibody is produced in rabbit after immunization with Algae Recombinant DNAK of Chlamydomonoas reinhardtii. The Antibody is tested and validated for WB assays with the following recommended dilutions: WB (1:5000). This DNAK antibody is unconjugated. |
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Antibody for Algae HSP70B (Plant) |
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| SPC-315D | Stressmarq | 0.1ml | EUR 366 |
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Description: A polyclonal antibody for HSP70 from Algae | Plant | Algae (Chlamydomonas reinhardtii) | Algae (Desmodesmus subspicatus) | Moss (Physcomitrella patens). The antibody is produced in rabbit after immunization with Algae Mature HSP70B protein expressed with N- and C- terminal hexahistidine tags in E.coli, purified with Ni-NTA. The Antibody is tested and validated for WB, IP assays with the following recommended dilutions: WB (1:10000). This HSP70 antibody is unconjugated. |
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Antibody for Algae HSP90C (Plant) |
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| SPC-317D | Stressmarq | 0.1ml | EUR 366 |
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Description: A polyclonal antibody for HSP90 from Algae | Algae (Chlamydomonas reinhardtii). The antibody is produced in rabbit after immunization with Algae C-terminal 238 amino acids of HSP90C expressed with N- and C-terminal hexahistidine tags in E.coli, purified with Ni-NTA.. The Antibody is tested and validated for WB, IP assays with the following recommended dilutions: WB (1:3000). This HSP90 antibody is unconjugated. |
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Cassettes in QuickLoad Sleeves |
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| M480-4SL-GREEN | ScyTek Laboratories | 1 ea. | EUR 284 |
Bromocresol Green |
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| GT4023-25G | Glentham Life Sciences | 25 g | EUR 110 |
Bromocresol Green |
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| GT4023-5G | Glentham Life Sciences | 5 g | EUR 54 |
Leucomalachite Green |
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| GT7606-10G | Glentham Life Sciences | 10 g | EUR 94 |
Leucomalachite Green |
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| GT7606-25G | Glentham Life Sciences | 25 g | EUR 158 |
Leucomalachite Green |
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| GT7606-5G | Glentham Life Sciences | 5 g | EUR 66 |
MitoView Green |
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| 70054 | Biotium | 20X50UG | EUR 226 |
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Description: Minimum order quantity: 1 unit of 20X50UG |
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Bromocresol green |
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| 20-abx186394 | Abbexa |
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Brilliant green |
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| BB0242 | Bio Basic | 25g | EUR 56.09 |
Leucomalachite green |
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| AG105 | Unibiotest | 1 mg | EUR 523 |
Malachite green |
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| AT060 | Unibiotest | 1mg | EUR 1790 |
Leucomalachite green |
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| AT105 | Unibiotest | 1mg | EUR 1368 |
Malachite green |
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| AG060 | Unibiotest | 1 mg | EUR 523 |
Thiazole Green, 10, 000X in DMSO |
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| 40086-0.5mL | Biotium | 0.5mL | EUR 188 |
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Description: Minimum order quantity: 1 unit of 0.5mL |
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Thiazole Green, 10, 000X in DMSO |
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| 40086-1mL | Biotium | 1mL | EUR 287 |
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Description: Minimum order quantity: 1 unit of 1mL |
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PhosLite™ Green |
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| 11630 | AAT Bioquest | 1 mg | EUR 219 |
Thiolite™ Green |
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| 21508 | AAT Bioquest | 5 mg | EUR 306 |
CytoTell™ Green |
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| 22253 | AAT Bioquest | 500 Tests | EUR 132 |
CytoTell™ Green |
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| 22254 | AAT Bioquest | 1000 Tests | EUR 176 |
LysoBrite™ Green |
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| 22643 | AAT Bioquest | 500 Tests | EUR 176 |
ViSafe Green Gel |
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| S435 | GeneOn | 500 µl | EUR 146 |
ViSafe Green Gel |
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| S435L | GeneOn | 5x500 µl | EUR 557 |
Green-DNA Dye |
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| SB2040 | Bio Basic | 100UL, 100UL | EUR 67.4 |
0.6ML MICROTUBES. GREEN |
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| MCT-060-G | CORNING | 1000/pk | EUR 183 |
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Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
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1.7ML MICROTUBES. GREEN |
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| MCT-175-G | CORNING | 500/pk | EUR 116 |
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Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
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Malachite green oxalate |
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| MD0330 | Bio Basic | 50g | EUR 57.83 |
Fast Green FCF |
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| FB0452 | Bio Basic | 5g | EUR 55.22 |
Fast Green FCF |
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| HY-D0914 | MedChemExpress | 500mg | EUR 108 |
Malachite Green [HRP] |
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| DAGA-073H | Creative Diagnostics | 1mg | EUR 1170 |
Leucomalachite Green [HRP] |
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| DAGA-074H | Creative Diagnostics | 1mg | EUR 1105 |
Malachite Green [BSA] |
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| DAGA-085B | Creative Diagnostics | 1mg | EUR 780 |
Leucomalachite Green [BSA] |
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| DAGA-086B | Creative Diagnostics | 1mg | EUR 780 |
Malachite Green [KLH] |
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| DAGA-091K | Creative Diagnostics | 1mg | EUR 1105 |
Leucomalachite Green [KLH] |
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| DAGA-092K | Creative Diagnostics | 1mg | EUR 975 |
Indocyanine green(Cardiogreen) |
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| E1KS1924 | EnoGene | 50 mg | EUR 247 |
Green Fluorescent Protein |
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| MO22184 | Neuromics | 100 ul | EUR 409 |
5G Bromocresol Green |
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| NAT1356 | National Diagnostics | 5G | EUR 156 |
10G Methyl Green |
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| NAT1364 | National Diagnostics | 10G | EUR 176 |
Acid Green 50 |
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| GT1171-100G | Glentham Life Sciences | 100 g | EUR 86 |
Acid Green 50 |
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| GT1171-25G | Glentham Life Sciences | 25 g | EUR 46 |
Acid Green 50 |
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| GT1171-500G | Glentham Life Sciences | 500 g | EUR 229 |
Reactive Green 5 |
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| GT6783-25G | Glentham Life Sciences | 25 g | EUR 126 |
Reactive Green 5 |
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| GT6783-5G | Glentham Life Sciences | 5 g | EUR 54 |
Green Fluorescent Protein |
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| CH22124 | Neuromics | 100 ul of 1 mg/ml | EUR 435 |
ViaVac Red/Green |
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| 29068 | Biotium | 100uL | EUR 261 |
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Description: Minimum order quantity: 1 unit of 100uL |
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BactoViewâ„¢ Live Green |
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| 40102 | Biotium | 100uL | EUR 320 |
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Description: Minimum order quantity: 1 unit of 100uL |
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Leuco- Malachiet green |
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| AG131 | Unibiotest | 1 mg | EUR 523 |
Green qPCR SuperMix |
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| 20-abx098031 | Abbexa |
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Fast Green FCF |
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| abx082188-5g | Abbexa | 5 g | EUR 203 |
Malachite green oxalate |
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| abx180007-25g | Abbexa | 25 g | EUR 272 |
C.I.Solvent Green 3 |
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| abx185369-25g | Abbexa | 25 g | EUR 509 |
Fast Green Solution |
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| FGN125 | ScyTek Laboratories | 125 ml | EUR 66 |
Fast Green Solution |
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| FGN500 | ScyTek Laboratories | 500 ml | EUR 96 |
Fast Green Solution |
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| FGN999 | ScyTek Laboratories | 1000 ml | EUR 124 |
Malachite green Antibody |
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| 48363-100ul | SAB | 100ul | EUR 333 |
Malachite green Antibody |
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| 48363-50ul | SAB | 50ul | EUR 239 |
Light Green Solution |
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| LGA030 | ScyTek Laboratories | 30 ml | EUR 59 |
Light Green Solution |
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| LGA125 | ScyTek Laboratories | 125 ml | EUR 66 |
Light Green Solution |
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| LGA3800 | ScyTek Laboratories | 1 Gal. | EUR 271 |
Light Green Solution |
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| LGA500 | ScyTek Laboratories | 500 ml | EUR 101 |
Light Green Solution |
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| LGA999 | ScyTek Laboratories | 1000 ml | EUR 140 |
DNASE & METHYL GREEN |
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| D04-102-10kg | Alphabiosciences | 10 kg | EUR 9392 |
DNASE & METHYL GREEN |
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| D04-102-2kg | Alphabiosciences | 2kg | EUR 2081 |
DNASE & METHYL GREEN |
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| D04-102-500g | Alphabiosciences | 500 g | EUR 602 |
Safe-Green™ |
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| G108-G | ABM | 1.0 ml | EUR 71 |
BRILLIANT GREEN AGAR |
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| B02-115-10kg | Alphabiosciences | 10 kg | EUR 1222 |
BRILLIANT GREEN AGAR |
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| B02-115-2Kg | Alphabiosciences | 2 Kg | EUR 305 |
BRILLIANT GREEN AGAR |
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| B02-115-500g | Alphabiosciences | 500 g | EUR 119 |
Malachite Green antibody |
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| 20-1184 | Fitzgerald | 100 ul | EUR 879 |
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Description: Sheep polyclonal Malachite Green antibody |
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Accuris qMax Green |
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| PR2000-H-100 | Benchmark Scientific | 1 PC | EUR 115.24 |
Accuris qMax Green |
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| PR2000-H-1000 | Benchmark Scientific | 1 PC | EUR 467.74 |
Accuris qMax Green |
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| PR2000-H-500 | Benchmark Scientific | 1 PC | EUR 284.31 |
Accuris qMax Green |
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| PR2000-H-S | Benchmark Scientific | 1 PC | EUR 73.77 |
Accuris qMax Green |
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| PR2000-L-100 | Benchmark Scientific | 1 PC | EUR 115.24 |
Accuris qMax Green |
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| PR2000-L-1000 | Benchmark Scientific | 1 PC | EUR 467.74 |
Accuris qMax Green |
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| PR2000-L-500 | Benchmark Scientific | 1 PC | EUR 284.31 |
Accuris qMax Green |
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| PR2000-L-S | Benchmark Scientific | 1 PC | EUR 73.77 |
Accuris qMax Green |
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| PR2000-N-100 | Benchmark Scientific | 1 PC | EUR 113.65 |
Accuris qMax Green |
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| PR2000-N-1000 | Benchmark Scientific | 1 PC | EUR 459.76 |
Accuris qMax Green |
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| PR2000-N-500 | Benchmark Scientific | 1 PC | EUR 279.53 |
Accuris qMax Green |
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| PR2000-N-S | Benchmark Scientific | 1 PC | EUR 72.98 |
Leucomalachite green-HRP |
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| 80-1192 | Fitzgerald | 500 ul | EUR 137 |
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Description: Leucomalachite green Conjugate for use in immunoassays |
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SYBR Green I |
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| B1747-25 | Biovision | EUR 1164 | |
SYBR Green I |
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| B1747-5 | Biovision | EUR 387 | |
ER-Hunt Green |
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| 9548-100 | Biovision | EUR 446 | |
Green Taq Mix |
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| P131-01 | Vazyme | 5 ml | EUR 119 |
Green Taq Mix |
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| P131-02 | Vazyme | 15 ml | EUR 150 |
Green Taq Mix |
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| P131-03 | Vazyme | 50 ml | EUR 248 |
Ribonuclease H, expressed in E. coli |
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| GZ5072-1KU | Glentham Life Sciences | 1 KU | EUR 500 |
Ribonuclease H, expressed in E. coli |
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| GZ5072-250U | Glentham Life Sciences | 250 U | EUR 213 |
Reverse Transcriptase, expressed in E. coli |
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| GZ8080-10KIU | Glentham Life Sciences | 10 KIU | EUR 285 |
Reverse Transcriptase, expressed in E. coli |
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| GZ8080-25KIU | Glentham Life Sciences | 25 KIU | EUR 540 |
Insulin (human) recombinant expressed in yeast |
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| B7407-10 | ApexBio | 10 mg | EUR 145 |
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Description: IC50: 5.01 nmol/L for insulin receptorInsulin is a peptide hormone produced by beta cells in pancreas. Insulin has been found to stimulate glucose uptake into muscle and adipose tissue, which is central to the maintenance of whole body glucose homeostasis. |
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MMP Green™ substrate |
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| 13520 | AAT Bioquest | 1 mg | EUR 480 |
Nuclear Green™ LCS1 |
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| 17540 | AAT Bioquest | 0.5 ml | EUR 132 |
Nuclear Green™ DCS1 |
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| 17550 | AAT Bioquest | 0.5 ml | EUR 132 |
Protonex™ Green 500 |
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| 21215 | AAT Bioquest | 1 mg | EUR 219 |
MitoLite™ Green EX488 |
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| 22675 | AAT Bioquest | 500 Tests | EUR 132 |
MitoLite™ Green FM |
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| 22695 | AAT Bioquest | 10x50 ug | EUR 176 |
MycoLight™ Green JJ98 |
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| 24000 | AAT Bioquest | 100 ul | EUR 219 |
MycoLight™ Green JJ99 |
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| 24001 | AAT Bioquest | 100 ul | EUR 219 |
Light green SF, Yellowish |
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| LD0007 | Bio Basic | 25g | EUR 75.23 |
Novel Green PLUS (20000X) |
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| LD003-0500 | GeneOn | 500 µl | EUR 172 |
Novel Green PLUS (20000X) |
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| LD003-0500L | GeneOn | 5x500 µl | EUR 731 |
Green Lysis Kit 50 |
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| GREENE1 | Next Advance | 50pack | EUR 156 |
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Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. |
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Green Lysis Kit 250 |
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| GREENE5 | Next Advance | 250pack | EUR 468 |
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Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. |
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80-Well Rack, Green |
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| R567-G | Bio Basic | 1 UNIT | EUR 52.51 |
Malachite Green ELISA Kit |
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| DEIA057 | Creative Diagnostics | 96T | EUR 1251 |
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Description: The Malachite Green ELISA Kit is a direct competitive enzyme immunoassay for the quantitative analysis of Malachite green in fish and shrimp samples. |
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Bromocresol green, sodium salt |
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| ED0087 | Bio Basic | 5g | EUR 63.92 |
Green Fluorescent Protein (GFP) |
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| MO22190 | Neuromics | 100 ul | EUR 409 |
25G Fast Green FCF |
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| NAT1348 | National Diagnostics | 25G | EUR 160 |
Green Fluorescent Protein (GFP) |
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| RA22134 | Neuromics | 100 ul of 1 mg/ml | EUR 409 |
Polycaspase Assay Kit, green |
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| KF17200 | Neuromics | 25 Tests | EUR 304 |
Bromocresol Green sodium salt |
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| GT3714-100G | Glentham Life Sciences | 100 g | EUR 229 |
Bromocresol Green sodium salt |
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| GT3714-25G | Glentham Life Sciences | 25 g | EUR 110 |
Bromocresol Green sodium salt |
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| GT3714-5G | Glentham Life Sciences | 5 g | EUR 54 |
Brilliant Green (C.I. 42040) |
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| GT6123-100G | Glentham Life Sciences | 100 g | EUR 78 |
Brilliant Green (C.I. 42040) |
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| GT6123-25G | Glentham Life Sciences | 25 g | EUR 46 |
Malachite green carbinol base |
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| GT7477-100G | Glentham Life Sciences | 100 g | EUR 96 |
Methyl Green (C.I. 42585) |
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| GT7495-100G | Glentham Life Sciences | 100 g | EUR 198 |
Methyl Green (C.I. 42585) |
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| GT7495-25G | Glentham Life Sciences | 25 g | EUR 94 |
Methyl Green (C.I. 42585) |
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| GT7495-5G | Glentham Life Sciences | 5 g | EUR 54 |
Malachite Green carbinol hydrochloride |
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| GT8088-100G | Glentham Life Sciences | 100 g | EUR 114 |
Malachite Green carbinol hydrochloride |
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| GT8088-25G | Glentham Life Sciences | 25 g | EUR 54 |
Green Fluorescent Protein (GFP) |
|||
| CH23105 | Neuromics | 400 ul | EUR 761 |
MitoView Green, Trial Size |
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| 70054-T | Biotium | 50UG | EUR 94 |
|
Description: Minimum order quantity: 1 unit of 50UG |
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Bromocresol green, free acid |
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| BD0159 | Bio Basic | 5g | EUR 63.92 |
Green qPCR SuperMix UDG |
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| 20-abx098032 | Abbexa |
|
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Top Green qPCR SuperMix |
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| 20-abx098033 | Abbexa |
|
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Tip Green qPCR SuperMix |
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| 20-abx098034 | Abbexa |
|
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Recombinant Green Fluorescent Protein |
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| 7-06112 | CHI Scientific | 10µg | Ask for price |
Recombinant Green Fluorescent Protein |
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| 7-06113 | CHI Scientific | 50µg | Ask for price |
Recombinant Green Fluorescent Protein |
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| 7-06114 | CHI Scientific | 1mg | Ask for price |
Trichrome Stain Solution (Green) |
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| TGG125 | ScyTek Laboratories | 125 ml | EUR 88 |
Trichrome Stain Solution (Green) |
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| TGG500 | ScyTek Laboratories | 500 ml | EUR 116 |
Trichrome Stain Solution (Green) |
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| TGG999 | ScyTek Laboratories | 1000 ml | EUR 143 |
Light Green Solution (0.1%) |
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| LGD-10000 | ScyTek Laboratories | 10 L | EUR 886 |
Light Green Solution (0.1%) |
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| LGD125 | ScyTek Laboratories | 125 ml | EUR 72 |
To verify the FC end result, we’ve got additionally used different experimental strategies for nuclear genome dimension estimation of the 2 strains. Curiously, the 2 strains had been discovered to have very distinct nuclear genome sizes. Our a number of traces of proof strongly point out that SAG 698-1a presumably had been confused with SAG 698-2 previous to 2005, and almost definitely represents Z. cylindricum or a intently associated species.